重症监护病房铜绿假单胞菌β-内酰胺酶检测与耐药特征分析  

Detection and analysis of beta-lactamases and antibiotic resistance on pseudomonas aerugionosa in intensive care unit

作　　者：徐伟红[1] 张正银[2] 

机构地区：[1]上海市长宁区同仁医院检验科,200050 [2]上海市长宁区中心医院检验科,200335

出　　处：《检验医学与临床》2011年第14期1692-1693,共2页Laboratory Medicine and Clinic

摘　　要：目的了解上海市长宁区中心医院重症监护病房铜绿假单胞菌β-内酰胺酶产生情况,为临床治疗及控制医院感染提供依据。方法用K-B法进行常规药敏试验,筛选出78株多重耐药铜绿假单胞菌,用改良三维试验检测各种β-内酰胺酶,同时用2-巯基丙酸抑制试验检测金属酶。结果 78株铜绿假单胞菌中产超广谱β-内酰胺酶(ESBLs)5株(6.4%)、产碳青酶烯酶(AmpC)31株(39.7%),17株同时产ESBLs和AmpC(21.8%),金属酶筛选出12株(15.4%)阳性。结论该院铜绿假单胞菌β-内酰胺酶以AmPC酶为主,临床应根据药敏试验结果,合理选用抗生素,以减少耐药菌株产生和控制医院感染。Objective To study the production of beta-lactamases on pseudomonas aeruginosa（PA） and provide the guideline for treatment and control of PA infection in intensive care unit（ICU）.Methods The susceptibility of 78 strains of PA was tested by K-B method.The three dimensional method was taken to test the various beta-lactamases.Results Among 78 strains,there were 5 strains producing extended spectrum beta-lactamases（ESBLs）,accounted for 6.4%,31 strains producing Ampc beta-lactamases（AmPC）,accounted for 39.7%,17 trains producing ESBLs and AmPC at the same time,12 strains of them were producing metallo-beta-lactamases.Conclusion The main beta-lactamases was AmPC beta-lactamases in our hospital.In order to reduce the drug-resistance stains and control the infection of PA,antibiotics should be used reasonably according to drug susceptibility test.

关 键 词：重症监护病房 铜绿假单胞菌 Β-内酰胺酶 

分 类 号：R378.99[医药卫生—病原生物学]