枣幼胚愈伤组织诱导和胚状体发生  

Callus Induction and Embryogenesis of Ziziphus jujuba

作　　者：张存智[1] 

机构地区：[1]宁夏职业技术学院,宁夏银川750002

出　　处：《北方园艺》2011年第13期125-127,共3页Northern Horticulture

摘　　要：以陇东马牙枣盛花后50～60d的幼胚为试材,采用三步培养法诱导胚状体。结果表明:诱导愈伤组织适宜的培养基为Nitsch培养基上附加BA 1.0mg/L+2,4-D 1.5mg/L+蔗糖30g/L+琼脂4.0g/L。诱导胚状体培养基宜选用,MS+BA 1.0mg/L+IBA 0.2mg/L+LH0.5mg/L+VC 0.1mg/L+蔗糖30g/L+琼脂4.0g/L,并获得完整植株。Young embryos of the cultivar ＇Longdongmayazao＇（Zizypthus jujuba）of 50-60 days after bilossom was induced to produced somatic embryos by three-step. The results showed that the proper medium of producing callus was Nitsch＋BA 1.0 mg/L＋ 2,4-D 1.5 mg/L＋sucrose 30 g/L＋agar 4. 0 g/L, the proper medium of producing somatic embryo form callus was MS＋ BA 1.0 mg/L＋ IBA 0. 2 mg/L＋ LH 0. 5 rag/L＋ vitamin C 0. 1 mg/L＋ sucrose 30 g/L＋LH 0. 5 mg/L＋vitamin C 0. 1 mg/L＋sucrose 30 g/L＋agar 4. 0 g/L. The medium of plantlet formation from somatiee embryo was MS＋BA 1.0 mg/L＋IAA 0.5 mg/L＋LH O. 5 mg/ L＋ vitamin C 0.1 mg/L＋ sucrose 30 g/L＋agar 4.0 g/L.

关 键 词：枣 愈伤组织 胚状体 

分 类 号：S665.103.6[农业科学—果树学]