鲤鱼Sox9基因HMG保守区的克隆及序列分析  

Cloning and Sequence Analyzing of Sox 9 Genes' HMG Section of C. carpio

作　　者：华慧颖[1] 王芳[1] 常重杰[2] 杜启艳[2] 

机构地区：[1]中州大学化工食品学院,河南郑州450044 [2]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南农业科学》2011年第7期147-151,共5页Journal of Henan Agricultural Sciences

摘　　要：为研究鲤鱼中与性别决定及骨组织发育相关基因Sox 9的特征及功能,从大量常见养殖鲤鱼品种及黄河野生鲤鱼个体中克隆得到了多个版本CcSox 9基因的HMG保守区序列,并对这些序列及国内外的其他鲤鱼Sox基因进行了启动子、多态性等序列分析。结果显示,鲤鱼中至少存在5个版本的活性Sox 9基因及1个Sox 9假基因。这些基因在编码序列中存在一定的多态性,在内含子序列中存在大量序列多态,并且活性基因中都存在至少1个启动子序列。假基因无内含子序列,在编码序列中存在多个终止密码子。To investigate the features and functions of Sox 9 genes which are related to sex-determination and bony-tissue-development in C.carpio,several CcSox 9 genes were cloned from large quantity of individuals of several usual C.carpio varieties and wild Yellow River C.carpio.Sequences,including promoter-sequences,polymorphism,of these CcSox 9 genes and other CcSox 9 genes cloned by other domestic and foreign areas were analyzed.The results revealed that at least 5 active CcSox 9 genes and a pseudo gene PCcSox 9 were found.Abundant polymorphisms,including sequence polymorphisms and length polymorphisms,were found in these genes＇ coding sequences,especially introns.At least one promoter sequence can be found in each active CcSox 9 gene＇s intron.Pseudo gene PCcSox 9 has no intron and several terminal codons were found in its coding sequence.

关 键 词：SOX9基因 鲤鱼 克隆 序列分析 

分 类 号：S965.116[农业科学—水产养殖]