2种蓼科植物体外抑菌试验研究  

Antibacterial Experiment of Two Polygonaceae Plants in vitro

作　　者：林燕文[1] 

机构地区：[1]惠州学院生命科学系,广东惠州516007

出　　处：《微生物学杂志》2011年第3期102-105,共4页Journal of Microbiology

摘　　要：为研究蓼科植物水蓼、火炭母体外抑菌效果,制备水煎煮液,测定其对枯草芽胞杆菌、大肠埃希菌、金黄色葡萄球菌、黑曲霉、青霉、酿酒酵母的抑菌圈直径、最小抑菌浓度及灭菌前后水煎煮液抑菌效果的变化。结果表明:当水蓼液浓度大于0.6 g/mL,火炭母液浓度大于0.2 g/mL时,2者对细菌的抑菌效果均依次为:金黄色葡萄球菌>大肠埃希菌>枯草芽胞杆菌。2种水煎煮液对3种真菌无抑制作用。火炭母液对3种细菌的最小抑菌浓度分别为1/8、1/16和1/64 g/mL,都比水蓼液的结果1/4、1/8和1/16 g/mL低。药液经121℃高压蒸汽灭菌处理后,水蓼液对3种细菌的抑制作用有所增强,火炭母液则对金黄色葡萄球菌的抑制作用有所增强。2种水煎煮液对3种细菌均有不同程度的抑制作用,可考虑以其为原料进行植物源抗菌剂的开发,拓宽其应用领域。Antibacterial effects of two species of Polygonaceae of Polygonum hydropiper（Ph） and Polygonum chinense（Pc） were studied in vitro with their water decoction,and determined their inhibitory rings,minimal inhibitory concentration（MIC） and the antibacterial effects before and after sterilization against Bacillus subtilis（Bs）,Escherichia coli（Ec）,Staphylococcus aureus（Sa）,Aspergillus niger（An）,Penicillium citrinum（Pc）,and Saccharomyces cerevisiae（Sc）.The results indicated that the antibacterial effects of these two extract were successively as follow： Sa Ec Bs when the concentration of these two decoctions were higher than 0.6 g/mL and 0.2 g/mL.These two extract had no antibacterial effects on the three fungi.The minimal inhibitory concentration of the decoctions from Pc against the three bacteria was 1 /8 g/mL,1 /16 g/mL,and 1 /64 g/mL,which were lower than that from Ph,i.e.1 /4 g/mL,1 /8 g/mL,and 1 /16 g/mL.The antibacterial effects of decoction from Ph against the three bacteria and that from Pc against Sa strengthened when they were treated under 121 ℃ high temperature.These two decoctions had different antibacterial effects on these three bacteria,therefore,the different degree of bacterial inhibition from Ph and Pc could be considered to develop and apply antibiotic agents from plant resources and widen their application fields.

关 键 词：蓼科植物 水蓼 火炭母 抑菌试验 最小抑菌浓度 

分 类 号：Q939.9[生物学—微生物学]