丙烯酰胺对大鼠神经行为和黑质纹状体多巴胺及其代谢产物含量的影响  

Effects of acrylamide on rat neurobehavior,the contents of dopamine and its metabolites in nigrostriatal dopaminergic pathways

作　　者：郭雄雄[1] 严汉英[2] 朱兰兰[1] 曾立爱[1] 熊飞严红 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,武汉430030 [2]武钢安全环保部安全环保研究所

出　　处：《卫生研究》2011年第4期441-444,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30771822)

摘　　要：目的初步探讨丙烯酰胺(ACR)对大鼠神经行为的影响,并通过测定黑质纹状体多巴胺(DA)及其代谢产物二羟苯乙酸(DOPAC)、高香草酸(HVA)含量阐明其可能机制。方法将健康成年雄性SD大鼠随机分成对照组和ACR组,每组7只。ACR组以40mg/kg的ACR灌胃染毒,对照组灌胃等容量的生理盐水,连续12天。第0、3、6、9和12天分别评定步态分值、后肢撑力及甩尾时间。末次给药后24小时,断头处死,于冰盘上迅速分离纹状体,HPLC荧光检测法检测DA及其代谢产物的含量。结果与对照组相比,ACR组大鼠的步态评分、后肢撑力指数显著性升高(P<0.01),甩尾时间显著降低(P<0.01)。纹状体内DA含量与对照组相比,约降低了65.64%,差异有显著性(P<0.01)。结论 ACR能引起大鼠神经行为功能的改变,同时能降低纹状体内DA含量,黑质纹状体DA系统可能参与了ACR的神经毒性作用。Objective To explore the neurotoxic effect of acrylamide on nigrostriatal dopaminergic pathways in male SD rats and its mechanism.Methods Fourteen male SD rats were randomly divided into 2 groups： control group,and ACR exposed group（40mg/kg）.Exposed SD rats were intragastrically administrated acrylamide（40mg/kg） once daily for 12 days,the control rats were only administrated with normal saline by gavage.Neurobehavioral indexes including body weight,gait scores,nervous sensory to heat,foot splay in hindlimb landing were measured,then the contents of dopamine（DA） and its metabolites,3,4-dihydroxyphenylacetic acid（DOPAC） and homovanilic acid（HVA） in the striatum were analyzed by HPLC-fluorescence detection.Results In comparison with control,there were significant differences in all neurobehavioral indexes（P0.05）.The contents of DA in ACR exposed group decreased by 65.64% compared with the control group（P0.05）.However,no significant differences of DOPAC and HVA content were found between ACR exposed group and control group.Conclusion Acrylamide may affect neurobehavior of rats and decrease the level of dopamine in striatum,thus induce a series of neurotoxic effects.

关 键 词：丙烯酰胺 多巴胺 纹状体 神经行为 

分 类 号：Q593.2[生物学—生物化学]