3种来源的野生斑节对虾携带病毒情况调查  

Investigation on status of carrying virus of wild Penaeus monodonfrom three resources

作　　者：杨丽诗[1] 黄建华[1] 孙苗苗[1,2] 周发林[1] 杨其彬[1] 温为庚[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2011年第4期546-552,共7页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(40976101);广东省自然科学基金项目(10451030002005817);国家现代农业产业技术体系(CARS-47);中央级公益性科研院所基本科研业务费专项资金项目(2009TS28);广东省自然科学基金(9151030002000008);渔业种质资源保护项目(2130135);广东省海洋渔业科技推广专项(A200901B02)

摘　　要：利用PCR方法,对2008年取自3个斑节对虾主产地(中国、泰国、非洲)的野生斑节对虾亲虾进行主要病毒病筛查。在所检测的210例野生亲虾样本中,均发现携带白斑综合征病毒(WSSV)、传染性皮下及造血组织坏死病毒(IHHNV)或斑节对虾杆状病毒(MBV)的阳性样本,其中泰国来源的斑节对虾亲虾WSSV携带率最高,达到89.8%,而中国来源的斑节对虾亲虾IHHNV携带率最高,为8.8%。12例样本同时检测出WSSV和IHHNV(阳性率为5.7%)。该结果揭示3种来源的斑节对虾亲虾普遍携带WSSV和IHHNV,其中WSSV仍然是感染斑节对虾的主要病原。调查结果增进了对斑节对虾主产区野生亲虾健康状况的了解,并在一定程度上揭示了WSSV感染的新规律。In our research,we used PCR technology to examine the viral pathogen of wild Penaeus monodon from three main producing areas,including China,Thailand,and Africa in 2008.In the 210 wild Penaeus monodon broodstocks,we detected the positive result that some of them were carrying white spot syndrome virus（WSSV）,infectious hypodermal and hematopoietic necrosis virus（IHHNV） or Monodon baculovirus（MBV）.The results showed that the penaeid shrimps from Thailand had a highest WSSV-carrying rate of 89.8%,and those from China had a highest IHHNV-carrying rate of 8.8%.Twelve samples carrying WSSV and IHHNV（positive rate is 5.7%） were detected.These results reveal the broodstocks from China,Thailand and Africa are ubiquitously infected by WSSV and IHHNV.Meanwhile,WSSV is still a major pathogen of penaeid shrimp.Our research enhanced the understanding of the healthy condition of wild Penaeus monodon broodstock from the three main producing areas,and also revealed the new pattern of WSSV infection to a certain degree.

关 键 词：野生斑节对虾 PCR检测 白斑综合征病毒 传染性皮下及造血组织坏死病毒 斑节对虾杆状病毒 混合感染 

分 类 号：S945.1[农业科学—水产养殖]