天津市病毒性腹泻的病原学分析  

Pathogenic Analysis on Viral Diarrhea in Tianjin

作　　者：田宏[1] 雷玥[1] 刘杨[1] 张颖[1] 李佳萌[1] 

机构地区：[1]天津市疾病预防控制中心病原生物检测所,天津300011

出　　处：《环境与健康杂志》2011年第7期605-608,共4页Journal of Environment and Health

基　　金：国家科技重大专项课题(2009ZX10004-209)

摘　　要：目的了解天津市病毒性腹泻的病原学分布情况。方法于2009年3—10月从天津市儿童医院和宝坻医院收集感染性腹泻患者的粪便标本,采用酶联免疫技术(ELISA)检测轮状病毒,采用逆转录-聚合酶链反应(RT-PCR)方法检测杯状病毒、星状病毒,并对轮状病毒ELISA检测阳性标本进行分型。采用聚合酶链反应(PCR)检测腺病毒。结果从天津市儿童医院收集儿童粪便标本124份,四种病毒检测阳性率顺位依次为杯状病毒(17.7%)>腺病毒(16.9%)>轮状病毒(12.1%)>星状病毒(1.6%),总检出率为48.4%。从宝坻医院收集成人粪便标本102份,四种病毒检测阳性率顺位依次为杯状病毒(8.8%)>腺病毒(3.9%)>轮状病毒(1.0%)>星状病毒(1.0%),总检出率为14.7%。16株轮状病毒G血清型阳性8株,G1为5株(31.2%),G3为3株(18.8%),8株未能分型(50%);P基因型分型结果阳性7株,P[4]为2株(12.5%),P[6]为1株(6.2%),P[8]为4株(25%),9株未能分型(56.3%)。G型与P型组合P[4]G1为1株(6.2%),P[8]G3为1株(6.2%),P[8]G1为3株(18.8%)。31株诺如病毒全部为GⅡ型。腺病毒主要是Ad40和Ad41型。星状病毒是1型。结论本次调查的该市儿童病毒性腹泻发病率明显高于成年人,病毒分布呈现出多样性。Objective To investigate the main pathogens of viral diarrhea in Tianjin. Methods The feces specimens of infectious diarrhea patients were collected from Tianjin Children＇s Hospital and Baodi Hospital. Rotavirus was detected by ELISA,calicivirus and astrovirus were detected by RT-PCR. Further strain characterization of rotavirus was carried out with RT-PCR and adenovirus were detected by PCR. Results Tested feces specimens with amount of 124 were collected from Tianjin Children＇s Hospital . Calicivirus was detected in 17.7% of the cases, adenoviruses in 16.9% , rotavirus in 12.1% and astrovirus in 1.6 %.The total positive rate was 48.4%. Tested feces specimens with amount of 102 were collected from Baodi Hospital. Calicivirus was detected in 8.8 % of the cases, adenoviruses in 3.9 %, rotavirus in 1.0 % and astrovirus in 1.0%.The total positive rate was 14.7%. Among 16 rotavirus positive samples, serotype GI （31.2%） was the predominant strain followed by G3 （18. 8 %） . 50 % of strains could not be typed. P genotyping showed P[8] （25%） was most common followed by P[4]（12.5%） and P[6]（6.2%）. 56.3 % of strains remained to be non-typeable. Strain P[8] G1 （18.8 %） was the most common combination followed by P[4]GI（6.2%） and P[8]G3 （6.2%）.All of 31 calicivirus were G Ⅱ genotype. Adenovirus 40 and 41 were predominant circulating genotype. Three Astrovirus were all 1 serotype. Conclusion The incidence of children viral diarrhea was higher than adults, and the diversity character was seen in the distribution of virus species and type.

关 键 词：病毒性腹泻 轮状病毒 腺病毒 儿童 成人 

分 类 号：R117[医药卫生—公共卫生与预防医学]