戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量测定  

Determination of Scopolamine Hydrobromide and Total Alkaloid in Jiedukang Tincture

作　　者：徐强[1] 章曙丹[1] 冯瑛[1] 

机构地区：[1]浙江省中药研究所,杭州310023

出　　处：《中国现代应用药学》2011年第7期673-676,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的测定中药戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量。方法采用Diamonsil C18柱(250 mm×4.6 mm,5μm)分离测定氢溴酸东莨菪碱,流动相:以甲醇-0.02 mol.L-1醋酸钠溶液(含0.02%三乙胺和0.3%四氢呋喃,用冰醋酸调pH到6.2)梯度洗脱;流速1 mL.min-1;检测波长:206 nm。采用酸性染料比色法测定戒毒康中的总生物碱,0.2%溴百里香酚兰溶液显色,420 nm处测得吸光度。结果氢溴酸东莨菪碱在1.0～5.0μg内呈良好的线性关系(r=0.999 6);平均回收率为98.8%,RSD为2.29%(n=6)。总生物碱在0.04～0.2 mg内呈良好的线性关系(r=0.999 8);平均回收率为97.6%,RSD为2.06%(n=6)。结论本方法简便、快速、准确、专属性强,可作为戒毒康酊剂的检验方法。OBJECTIVE To establish an HPLC method for determination of contents of scopolamine hydrobromide and total alkaloid in Jiedukang tincture. METHODS A Diamonsil C18 column（250 mm×4.6 mm, 5μm） was used to isolate and determine scopolamine hydrobromide. The mobile phase was methanol-0.02 mol.L^-1 NaAc solution （with 0.02% triethylamine and 0.3% tetrahydrofuran, adjusted to pH 6.2 by HAc）. The flow rate was 1 mL.minH. The detection wavelength was set at 206 nm. Total alkaloids in Jiedukang tincture were determined with acid dye colorimetry. 0.2% Bromothymol blue was used as the dye reagent and the wavelength was set at 420 nm. RESULTS The linear range of scopolamine hydrobromide was 1.0-5.0 μg with a good correlation （r=0.999 6）. The average recoveries was 98.8% with RSD of 2.29%（n=6）. The total alkaloids showed good linearity within the range of 0.04-0.2 mg（r=0.999 8）, the average recoveries was 97.6% with RSD of 2.06%（n=6）.

关 键 词：氢溴酸东莨菪碱 高效液相色谱法 总生物碱 

分 类 号：R943.3[医药卫生—药剂学]