清开灵注射液对人血清补体和RBL-2 H3细胞影响的体外研究  

Effect of Qingkailing injection(QKLI) on complement and RBL-2 H3 cells in virto

作　　者：陈莉婧[1] 廖国平[1] 汪艳[1] 贺帅[1] 张忠义[1] 

机构地区：[1]南方医科大学珠江医院,广东广州510282

出　　处：《中国中药杂志》2011年第14期1884-1888,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(90709046);广东省科技计划项目(2007B31402006)

摘　　要：目的:体外研究清开灵注射液对人血清补体和RBL-2 H3细胞的影响。方法:采用ELISA法检测清开灵注射液对人血清补体末端复合物SC5 b-9含量的影响。将不同批号、不同浓度的清开灵注射液与RBL-2 H3细胞共孵育,作用45 min后,显微镜下观察细胞脱颗粒,显色法测定β-氨基己糖苷酶释放度和ELISA法测定组胺释放量。结果:清开灵注射液能使人血清中SC5 b-9含量降低,与PBS组比较,差异有统计学意义(P<0.05);能刺激RBL-2 H3细胞释放组胺和β-氨基己糖苷酶,与阴性组比较,差异具有统计学意义(P<0.05)。结论:清开灵注射液不能引起补体系统激活,可以直接刺激肥大细胞脱颗粒释放组胺和β-氨基己糖苷酶。其在临床引起的不良反应可能为非补体激活相关的类过敏反应。Objective： To investigate the effect of Qingkailing injection（QKLI） on complement and RBL-2 H3 cells in virto. Method： The mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. β-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA. Result： The QKLI can reduce the content of SC5 b-9 （P〈0. 05） and promote the release of β-heosaminidase and histamine significantly （P〈0. 05）. Conclusion： QKLI didn＇t induce the complement activation, but induced the release of β-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.

关 键 词：清开灵注射液 补体系统激活 肥大细胞脱颗粒 类过敏 

分 类 号：R286[医药卫生—中药学]