通江百合天然群体遗传多样性的SRAP分析  

Genetic diversity analysis of natural groups of Lilium sargentiae by SRAP markers

作　　者：智丽[1] 滕中华[2] 刘旭[3] 李名扬[1] 

机构地区：[1]西南大学园艺园林学院重庆市花卉工程技术研究中心南方山地园艺学教育部重点实验室,重庆400715 [2]西南大学农学与生物科技学院,重庆400715 [3]重庆市药物种植研究所,重庆408435

出　　处：《中国中药杂志》2011年第14期1921-1926,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30872063);中央高校基本科研业务费专项资金项目(XDJK2010C077)

摘　　要：目的:采用SRAP标记技术对通江百合天然群体遗传多样性进行研究。方法:通过SRAP标记对通江百合10个天然群体100个个体遗传多样性进行分析,利用POPGENE 1.32和NTSYS-pc进行数据分析。结果:选择15对引物共扩增出170个位点,多态位点163个,种水平上多态位点百分率为90.58%,Ne i′s基因多样度指数H0.263 1,Shannon′s信息指数I0.366 1;遗传分化系数Gst0.367 2,遗传距离变化范围为0.202 1～0.574 9。结论:通江百合物种水平上的遗传多样性较高;居群内遗传多样性大于居群间遗传多样性,变异主要存在于居群内部;聚类结果与地理分布表现出明显相关性。Genetic diversity of Lilium sargentiae was detected in this paper by sequence-related amplified polymorphism （SRAP） marker. One hundred wild samples were collected from 10 places, and 15 SRAP primer combinations were used for determination. NTSYS-pc2.1 and POPGEN1.32 were used for data analysis. The results showed that a total of 170 clear DNA bands were amplified, 163 of which were polymorphic. The proportion of polymorphic loci was 90.58% on the level of species. Nei＇s （1 973） gene diversity （H） was 0.263 1, Shannon＇s Information index was 0.366 1, the Gst was 0.367 2, and the genetic distance ranged from 0.202 1 to 0.574 9. All materials could be clustered into four groups by UPGMA. The results demonstrated that the genetic diversity of L. sargentiae was rich on the level of species, and the genetic diversity within populations exceeded among populations. The correlations of genetic diversity and distribution were significant in L. sargentiae.

关 键 词：通江百合 SRAP 遗传多样性 

分 类 号：S644.1[农业科学—蔬菜学]