马钱子总碱囊泡的制备与评价  

Preparation and characterization of niosomes of Semen Strychni alkaloids extract

作　　者：胡杰 吴珍珍 胡克菲 张卫华 张保献 

机构地区：[1]北京因科瑞斯生物制品研究所,北京102209

出　　处：《中国中药杂志》2011年第14期1955-1958,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-395)

摘　　要：目的:制备高包封率的马钱子总碱囊泡。方法:采用pH梯度法,以包封率为主要考察指标,优选马钱子总碱囊泡的处方组成及制备工艺,并进行验证评价。结果:以司盘60-胆固醇1∶1为囊材,药物-囊材1∶10,柠檬酸溶液为水相,用碳酸钠调节pH梯度大于4.0,温度50℃时,可制得马钱子总碱囊泡,粒径179.2 nm,Zeta电位-25.41 mV,平均包封率86.9%;结论:pH梯度法适合于制备马钱子总碱囊泡。Objective： To prepare a noisome formulation of Semen Strychni alkaloids extract with high encapsulation efficiency. Method： S. Strychni alkaloids were encapsulated into niosomes by pH gradient loading method. The factors influencing on the encapsulation efficiency were investigated, and formulation and preparation process of niosomes were optimised and validated. Result： When the drug to lipid weight ratio was under 1 ∶ 10, pH gradient in buffer solution of citric acid at 50 ℃ was more than 4. 0, the niosomes （mean diameter 179. 2 nm and Zeta potential -25. 41 mV） were formed with encapsulation efficiency of over 86. 9%. Conclusion： The pH gradient loading method was reliable for preparing niosomes of Semen Strychni alkaloids extract.

关 键 词：马钱子总碱 囊泡 包封率 PH梯度法 

分 类 号：R283[医药卫生—中药学]