HPLC-ELSD内标法测定8个产地黄芪药材、饮片中黄芪甲苷的含量  

Determination of astragaloside Ⅳ of eight area in Astragali Radix is by HPLC-ELSD internal standard method

作　　者：裴彩云 王宗权 贾继明 宋剑 

机构地区：[1]河北以岭医药研究院,河北石家庄050035

出　　处：《中国中药杂志》2011年第14期1982-1984,共3页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2005CB523301);国家"十一五"科技支撑计划项目(2006BAI08B04-09)

摘　　要：目的:建立HPLC-ELSD内标法,测定黄芪药材中黄芪甲苷含量。方法:采用人参皂苷Rb2为内标物,Agilent TC-C18(4.6 mm×250 mm,5μm)色谱柱,甲醇-水(72∶28)为流动相,流速1 mL.min-1,柱温30℃,ELSD检测器漂移管温度为75℃,以洁净干燥的压缩空气为雾化气体,压力为172.4 kPa。结果:黄芪甲苷在进样量0.562 4～5.624μg,进样量的常用对数与对照品峰和内标峰峰面积比值的常用对数成良好线性关系(r=0.999 9);平均回收率为98.06%,RSD为0.98%。结论:建立的内标法准确度高,重复性好,是控制黄芪药材质量的较理想方法。Objective： To establish an HPLC-ELSD internal standard method for determination of astragaloside Ⅳ in Astragali Radix.Method： With Ginsenoside Rb2 as internal standard, the separation were carried out on an Agilent TC-C18 （4.6 mm×150 mm, 3.5 μm） column with methanol-water （72∶28） as mobile phase. The flow rate was 1.0 mL·min-1 and the drift tube temperature of the ELSD was 75 ℃. The gas pressure was set at 172.4 kPa using the clean and dry compressed air as spray gas. Result： There was good linearity in the range of 0.5624-5.624 μg of astragaloside Ⅳ （r=0.999 9）;The average recovery was 98.06% with RSD of 0.98%. Conclusion： The internal standard method is accurate and reproducible, and suitable for quality control of radix astragali.

关 键 词：高效液相蒸发光检测 内标法 黄芪 黄芪甲苷 

分 类 号：R284.1[医药卫生—中药学]