侵袭性烟曲霉菌病大鼠模型的建立及分析  

Establishment and analysis of murine model of invasive pulmonary aspergillosis

作　　者：张常然[1] 邢艳利[1] 崔卫玲[1] 牛媛媛[1] 林建聪[1] 

机构地区：[1]中山大学附属第一医院黄埔院区呼吸内科,广州市510700

出　　处：《实用医学杂志》2011年第15期2704-2707,共4页The Journal of Practical Medicine

基　　金：广东省科技计划资助项目(编号:2009B030801118)

摘　　要：目的:建立侵袭性肺烟曲霉菌病大鼠模型,并对模型研究价值进行相关分析,为侵袭性肺曲霉菌病的实验研究提供参考。方法:腹腔注射环磷酰胺,动态观察大鼠血常规变化,建立免疫抑制大鼠模型。自制气管插管接种烟曲霉菌孢子悬液引起侵袭性肺烟曲霉菌病,分别于接种后1、3、5、7d处死大鼠后取肺组织进行病理切片观察及组织匀浆培养。结果:分次不同剂量环磷酰胺腹腔注射后大鼠表现为精神萎靡、体重减轻,血常规示白细胞及分类细胞数量明显减少,中性粒细胞、淋巴细胞不能分类。病理切片可见免疫抑制大鼠感染烟曲霉菌后随着时间延长肺组织病变逐渐加重。肺组织出血、纤维素渗出;烟曲霉菌孢子聚集并长出菌丝,引起肺泡结构破坏、弥漫性肺水肿。结论:成功建立了免疫抑制大鼠侵袭性肺烟曲霉菌病动物模型,为研究免疫抑制宿主侵袭性肺曲霉菌病的发病机制、病理生理、药物疗效提供参照。Objective To establish a murine model of invasive pulmonary aspergillosis and to analyze its reserch value. Methods Mice were immunosuppressed with cyclosphosphamide by intraperitoneal injections and blood sample were monitored. Invasive pulmonary aspergillosis was induced by inoculation of Aspergillus fumigatus spore suspension with self-made tracheal intubation. Mice were sacrificed on day 1,3,5,7 after inoculation and their lung tissues were was cultured and histopathologically analyzed. Results Mice shows a loss of weight and depression after a series of different dose of cyclosphosphamide injection. Routine blood test shows a significant decrease of white blood cells . Nutrogranulocytes and lymphocytes can＇t be distinguished. By observing pathological slices, we can see lung tissue lesion aggrates gradually after inoculation. Haemorrhage and cellulose exsudation is obviously seen; sporus aggregat and germinate. As a resuh, alveoli are destroyed diffusely and a large area of lung edema happened. Conclusions A murine model of invasive pulmonary aspergillosis is succes sfully established, which will de a reference of further studies of aspergillosis pathogenesis and pathophysiology, in addition to antifungal agent pharmaeodynamics.

关 键 词：侵袭性肺烟曲霉菌病 免疫抑制 血常规 组织病理学检查 

分 类 号：R519[医药卫生—内科学] R-332[医药卫生—临床医学]