褪黑素与酵母硒联合应用对肿瘤细胞生长的抑制作用  

Inhibiting effect of melatonin combined with yeast selenium on tumor cell growth

作　　者：王伟迪[1] 徐维平[1,2] 魏伟[1] 路景涛[1] 王杰[1] 周冉[1] 黄莺[1] 魏浩洁[1] 

机构地区：[1]安徽医科大学临床药理研究所,省部共建抗炎免疫重点实验室,安徽省中药研究与开发重点研究室,抗炎免疫药物工程技术研究中心,合肥230032 [2]安徽医科大学附属省立医院,合肥230001

出　　处：《中国新药杂志》2011年第14期1334-1340,共7页Chinese Journal of New Drugs

摘　　要：目的:观察与相同剂量的单药相比,褪黑素(MT)与酵母硒(Se)联合应用是否能提高对肿瘤的治疗效果及其机制。方法:将S180肉瘤细胞接种于小鼠右腋皮下,分为7组:模型组、Se 100μg.kg-1组、Se200μg.kg-1组、MT40 mg.kg-1组、MT80 mg.kg-1组、Se 100μg.kg-1+MT40 mg.kg-1组、环磷酰胺(CTX)组。10 d后处死小鼠。测定肿瘤组织内细胞周期蛋白依赖性激酶2(CDK2)和p53的表达水平。结果:MT与Se联合应用可显著提高抑瘤率,降低肿瘤重量,降低CDK2的表达水平,提高p53和p21的表达水平。结论:与相同剂量的单药治疗肿瘤相比,MT与Se两者联合应用能明显提高疗效。Objective： To determine whether the antitumor effect of melatonin（MT） combined with selenium（Se） is superior to the same dose of MT or Se alone,and its mechanism.Methods： S180 sarcoma cells were inoculated subcutaneously on the right axilla of male mice.The mice were randomized into 7 groups,and treated with Se 100 and 200 μg·kg-1,MT 40 and 80 mg·kg-1,Se 100 μg·kg-1＋MT 40 mg·kg-1,or cyclophosphamide（CTX）.At 10 days after inoculation,the mice were euthanized.The expression of cyclin-dependent kinase 2（CDK2） and p53 in tumor tissues was detected.Results： Both Se and MT reduced the growth of S180 sarcoma cells.Their antitumor effects were mediated via decreasing CDK2 expression and increasing the expression of wild-type p53.The effect of MT combined with Se was more evident.Conclusion： MT and Se are promising drugs for tumor treatment,and their combination can potentiate the efficacy.

关 键 词：褪黑素 硒 细胞周期 CDK2 P53 

分 类 号：R979.1[医药卫生—药品]