新生大鼠海马神经元的改良培养  

MPROVED CULTURE OF HIPPOCAMAL NEURONS OF NEONATAL RAT

作　　者：高小青[1] 杜杰[2] 邓莉[1] 郭侃[1] 杨朝鲜[1] 王特为[1] 

机构地区：[1]泸州医学院医学基础研究中心,四川泸州646000 [2]泸州医学院教务处,四川泸州646000

出　　处：《泸州医学院学报》2011年第3期228-230,共3页Journal of Luzhou Medical College

基　　金：四川省教育厅项目(2006A155);四川省科技厅项目(2006J13-005-1)

摘　　要：目的:探讨体外培养高纯度神经元的方法。方法:体外培养新生大鼠海马神经干细胞,再使神经干细胞在无血清N2添加剂培养基条件下向神经元方向分化。结果:从新生大鼠海马组织培养出的神经干细胞,表达巢蛋白,神经干细胞的分化细胞绝大部分表达MAP2,极少数几个细胞表达GFAP。结论:通过本实验方法可获得高纯度的神经元,为后续实验奠定基础。To investigate the method of obtaining high content of neuron in vitro.Methods： Hip-pocamal neural in serum-free pressed nestin, pressed GFAP. periment,which stem cells（NSCs） of neonatal rat were cultured in vitro, and then differentiated to neuron direction medium containing N2 supplement. Results： The NSCs from hippocampus of neonatal rat ex- and most differentiated ceils of NSCs expressed MAP2,sporadically several differentiated cells ex- Conclusion： Highly purified neuron can be obtained by the method adopted by authors in the ex- underlies luther investigation.

关 键 词：细胞培养 神经干细胞 神经元 大鼠 

分 类 号：R338[医药卫生—人体生理学]