D1S80座位的等位基因扩增片段长度多态性分析  

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF ALLELES OF LOCUS D1S80

作　　者：黎旭[1] 赵崇[1] 张镜宇[1] 

机构地区：[1]天津医科大学内分泌研究所

出　　处：《天津医科大学学报》1999年第3期10-12,共3页Journal of Tianjin Medical University

摘　　要：目的：对天津地区１１２ 名无关个体的Ｄ１Ｓ８０ 基因座位进行了扩增片段长度多态性分析，检测其等位基因和基因频率。方法： 应用聚合酶链反应，小型聚丙烯酰胺凝胶电泳和银染方法观察长度多态性。结果：发现了２６ 个等位基因，大小在３５５～７７１ｂｐ 之间，基因频率为０．００４５～０．１３８４，杂合度为７３％ 。７０ 种基因型，个人鉴别力为０．９８５。对６ 个家系２３ 名相关个体的分析结果符合孟德尔定律。结论： Ｄ１Ｓ８０ 基因座位个人鉴别力高，ＰＣＲ方法简便、灵敏。Objective: The amplified fragment length polymorphism (Amp-FLP) of locus D1S80 among 112 unrelated TianJin chinese residents was analysed. Methods: This analysis was performed with polyacrylamide mini-gel electrophoresis followed by the silver staining. Results: 26 alleles, ranging from 355 to 771 bp in size and from 0.0045 to 0.1384 in frequency, and 70 genotypes were observed. The heterozygosity was 73% , and the discrimination power (DP) was 0.98 . The study on six families demonstrated that this locus was inherited according to Mendelian law. Conclusion: The technique used here is quite simple, rapid, extremely sensitive, and does not require the use of radioisotopes. It could provide a powerful new tool for the forensic idendification of individuals and paternity testing.

关 键 词：D1S80 基因 座位 PCR 扩增片段长度 多态性 

分 类 号：D919.2[医药卫生—法医学] Q78[政治法律—法学]