纳米与常规二氧化钛对A549细胞毒性及DNA损伤的比较  

Comparative Study of Cytotoxicity and DNA Damage Induced by Nano-and Micro-TiO_2 Particles on A549 Cells in Vitro

作　　者：洪丽玲[1] 丁树娟[1] 朱江波[1] 朱玉平[1] 张天宝[1] 

机构地区：[1]第二军医大学基础部卫生毒理学教研室,上海200433

出　　处：《环境与职业医学》2011年第7期393-397,共5页Journal of Environmental and Occupational Medicine

基　　金：国家重大科学计划子课题(编号:2006CB932505);军特药专项课题(编号:2008ZXJ09014--0095);上海市公共卫生重点学科建设项目(编号:08GWZX0301)

摘　　要：[目的]比较纳米二氧化钛(nano-TiO_2)和常规TiO_2对细胞的毒性及DNA损伤有无差异。[方法]体外培养人肺腺癌细胞(A549细胞),采用四甲基偶氮唑盐比色法(MTT)和单细胞凝胶电泳技术(彗星实验)比较25、50、100、200μg/mL质量浓度(以下简称"浓度")的nano-TiO_2(5～10nm)和常规TiO_2的细胞毒性与DNA损伤作用。[结果]nano-TiO_2对A549细胞的毒性与常规TiO_2的变化趋势相近,nano-TiO_2毒性大于常规TiO_2,200μg/mL浓度时作用12h以上两者细胞存活率的差异有统计学意义(P<0.05)。与对照组相比,nano-TiO_2各剂量组尾DNA含量、Olive尾距、彗星尾长均明显增加(P<0.05);常规TiO_2在受试剂量下尾DNA含量、Olive尾距、彗星尾长与对照组比较均无明显增加。[结论]nano-TiO_2对A549细胞的毒性高于常规TiO_2,且nano-TiO_2可诱导DNA损伤,而常规TiO_2未观察到DNA损伤作用。表明nano-TiO_2与常规TiO_2在毒性大小和毒作用性质均有差异。[ Objective ] To compare the difference between nano-and micro-TiO2 particles in their cytotoxicity and DNA damage effects on A549 cells. [ Methods ] A549 human lung adenocarcinoma cells were exposed to different concentrations of these two particles （ 0, 25, 50, 100 and 200 μg/mL ）separately, the methyl thiazolyl tetrazolium （ MTT ）assay and the single cell gel electrophoresis （ comet assay ） were applied to measure the cytotoxicity and DNA damage. [ Results ] It showed that the developed trend of the cytotoxicity induced by nano-TiO2 and micro-TiO2 was similar, but at the same level, nano-TiO2 induced greater damage than the micro-TiO2. For example, after 12 h treatment, nano-TiO2 in a dose of 200 μg/mL inhibited the viability of A549 cells significantly compared with the micro-TiO2 group. In addition, compared to the control group, the tail DNA content, Olive tail moment and comet tail length of the nano-TiO2 group increased significantly （ P〈0.05 ）; whereas these indices in micro-TiO2 group did not change. [ Conclusion ] Our data demonstrates that nano-TiO2 particles possess a toxic potential towards A549 cells which may be significantly higher than micro ones; the toxic effects occur earlier in time and lower in dose compared with micro-TiO2, and nano-TiO2 particles can induce DNA damage, but micro-ones do not. It is shown that the nano- and micro-TiO2 particles have some differences in toxicity and toxic properties.

关 键 词：纳米二氧化钛 常规二氧化钛 A549细胞 细胞毒性 DNA损伤 

分 类 号：R114[医药卫生—卫生毒理学]