水稻脯氨酸代谢关键酶对水分胁迫的响应  

Responses of the key enzymes involved in proline metabolism in rice seedling under water stress

作　　者：赵贵林[1] 陈强[1] 胡国霞[1] 褚妍[1] 马莲菊[1] 李雪梅[1] 

机构地区：[1]沈阳师范大学化学与生命科学学院,辽宁沈阳110034

出　　处：《干旱地区农业研究》2011年第3期80-83,共4页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金资助课题(30870205;31070285);辽宁省教育厅资助课题(L2010516);辽宁省自然科学基金(20092070;20102205);沈阳师范大学博士后资助课题

摘　　要：以二叶期水稻幼苗为试验材料,研究其在15%PEG6000模拟的水分胁迫下脯氨酸积累及代谢关键酶活性变化,结果表明:叶片和根系中脯氨酸含量在胁迫后快速积累,胁迫24 h达到最大值,随后逐渐降低;控制脯氨酸合成的关键酶吡咯啉-5-羧酸合成酶(P5CS)和鸟氨酸转氨酶(δ-OAT)活性都呈现先升后降的变化趋势,而控制脯氨酸降解的关键酶脯氨酸脱氢酶(ProDH)活性呈现先降后升的变化趋势。Using rice seedlings at two-leaf stage as tested materials,the free proline content and activities of enzymes involved in proline metabolism were studied under water stress induced by 15%PEG.The results showed that the proline content increased obviously under stress,reached the maximum after 24 h and then decreased.The activities of △1-pyrroline-5-carboxylate synthetase（P5CS） and ornithine-δ-aminotransferase（δ-OAT）,the key enzymes of proline accumulation,increased significantly and then declined;while the activity of proline dehydrogenase（ProDH）,a key enzyme of proline degradation,declined significantly and then increased.

关 键 词：水分胁迫 脯氨酸 吡咯啉-5-羧酸合成酶 鸟氨酸转氨酶 脯氨酸脱氢酶 

分 类 号：S511[农业科学—作物学] Q945.78[生物学—植物学]