利用荧光SSR标记鉴别杨树品种  

Identification of Poplar Varieties by SSR Markers Using Capillary Electrophoresis with Fluorescence Detection

作　　者：冯锦霞[1] 张川红[1] 郑勇奇[1] 孙荣喜[1] 

机构地区：[1]中国林业科学研究院林业研究所国家林业局植物新品种分子测定实验室国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学》2011年第6期167-174,共8页Scientia Silvae Sinicae

基　　金：国家林业局"948"引进项目"林木新品种测试技术引进"(2001-21);植物新品种分子测试技术指南(2007011)

摘　　要：世界上天然杨树约100多种,我国有50多种。杨树作为重要造林树种,多用于水土保持、防风防沙和园林绿化,也是造纸、建筑及木材工业的重要经济树种。杨树易繁殖,生长速度快,生产周期短,在我国广泛栽培,我国也是世界上杨树人工林面积最大的国家（Carleetal.,2005）。我国杨树引种和育种的历史可追溯到新中国建立时期,杨树种质资源十分丰富,我国已培育了许多优良杨树品种（马常耕,1995）。随着育种技术的发展,新品种产生的速度与日俱增。然而,由于杨树品种繁多,形态相似,容易出现品种混淆。特别是近年来杨树种苗市场的迅速发展,为了追逐经济效益,杨树种苗市场上出现了以次充好。SSR markers were used to identify 14 poplar varieties, including 4 varieties of Populus deltoides, 2 varieties of Populus nigra and 3 transgenic varieties of the same species, 2 hybrid varieties, ‘Senhai 1’ and ‘Senhai 2’, of P. deltoides ‘55/65’ × P. cathayana and 2 hybrid varieties, ‘Danhong’ and ‘Juba’, of P. deltoides ‘55/65’ × P. deltoides‘2KEN8’, by using capillary electrophoresis with fluorescence detection. A total of 58 primers were screened to detect differences of SSR markers among the varieties. The 4 varieties of P. deltoides were distinguished by 4 primers, the 5 varieties of P. nigra were distinguished by 5 primers, and the hybrid varieties of the 2 crosses were identified by 4 primers. Varieties of Aigeiros and Tacamahaca displayed their own unique peaks in 4 SSR loci （GCPM_1574, GCPM_1155, GCPM_1293 and GCPM_1502）. P. deltoides and P. nigra were found significantly different at the SSR loci. There were different SSR genotypes between the transgenic and non-transgenic varieties of P. nigra. The results indicated that the SSR markers are reliable for identifying those poplar varieties including transgenic varieties. The pedigree analysis of poplar hybrids revealed that the hybrid variety ‘Danhong’had 2 peaks of 109 bp and 129 bp at 2 SSR loci （GCPM_1037 and GCPM_1376） that did not exist in its parents, indicating possible pollen contamination occurred during the pollination process.

关 键 词：杨树 SSR 毛细管电泳 荧光检测 品种鉴别 

分 类 号：S718.49[农业科学—林学] S718.46