蚯蚓纤溶酶壳聚糖微球的制备及性质  

Preparation of Chitosan Microsphere Loaded Earthworm Fibrinolytic Enzymes and Its Properties

作　　者：武金霞[1] 王英锐[1] 田志鹏[1] 李瑞[1] 张贺迎[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北大学生物技术研究中心,河北保定071002

出　　处：《河北大学学报（自然科学版）》2011年第4期406-412,共7页Journal of Hebei University(Natural Science Edition)

基　　金：河北大学博士基金资助项目

摘　　要：以蚯蚓纤溶酶为生物大分子模式药物,研究壳聚糖载药微球的制备方法及对药物活性和稳定性的影响.以壳聚糖为原料,采用超声乳化交联法制备壳聚糖微球,分别吸附和包埋蚯蚓纤溶酶.在油水体积比15/1,搅拌转速800 r/min,1 mL戊二醛作为交联剂,超声处理5 min条件下乳化交联制备微球,包埋法具有较高的载药率和包封率,分别达到52.6%和48.7%,具有一定的缓释效果,在72 h药物累计释放率约为65%.用生理盐水溶出微球中的蚯蚓纤溶酶,电泳图谱显示,多数组分能被溶出,条带没有变化,在72 h内,包埋法活性总溶出率达51.5%.To study the preparing methods of chitason microspheres and investigate the influence in drug activity and stability when earthworm fibrinolytic enzymes （EFEs） was as biomacromolecule model drug. The chitosan microspheres were prepared by ultrasonic emulsification method, EFEs was encapsulated and adsorbed into microspheres respectively. The optimized factors were as follows. V（O）/V（W） was 15/1 ,stirring speed was 800 r/rain, adding 1 mL of glutaraldehyde, ultrasonic treatment for 5 rain. The loaded rate and encapsulation efficiency of the microsphere encapsulated EFEs at these conditions were 52.6% and 48.7% respectively, the microsphere pocessed the sustained releasing property, the total releasing rate in 72 hours were about 65%. Physiological saline was used to dissolve the EFEs from microsphere, the photogram of PAGE indicated that most of the EFEs components could be dissolved, and no obvious changes could be observed. The dissolved EFEs activity was determined by Folin-phenol method, the results showed that the total dissolution rate of the microsphere encapsulated EFEs was 51.5 % when dipped in physiological saline for 72 hours.

关 键 词：蚯蚓纤溶酶 壳聚糖微球 载药率 包封率 溶出率 

分 类 号：Q55[生物学—生物化学]