三种炎症因子在致炎大鼠乳腺组织中的表达  

Expression of three cytokines in rat inflammatory mammary tissue

作　　者：杨颖[1] 王俊杰[1] 崔永勇[1] 胡格[1] 杨佐君[1] 

机构地区：[1]北京农学院兽医学中医药北京市重点实验室,北京102206

出　　处：《北京农学院学报》2011年第3期28-30,共3页Journal of Beijing University of Agriculture

基　　金：北京市教育委员会科技计划面上项目(KM200910020007)

摘　　要：为探明在酯多糖（LPS）致炎的大鼠乳腺中,黏附因子-1（ICAM-1）、肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）表达分布的变化。取产后5 d乳房注射LPS致炎大鼠的乳腺组织,及LPS致伤的体外培养大鼠乳腺MVECs（Microvascular Endothelial Cells）,采用免疫组化方法检测ICAM-1、TNF-α和IL-1β的表达。结果表明：LPS处理大鼠乳腺后4~24 h内,乳腺组织切片试验和体外培养大鼠乳腺微血管内皮细胞（Rat MammaryMicrovascular Enclothelial Cells,RMMVECs）试验中ICAM-1和IL-1β的表达逐渐增强,TNF-α在乳腺组织切片试验中6 h表达显著增强,之后则逐渐减弱。在体外培养RMMVECs中-α表达逐渐增强。表达部位主要在乳腺上皮细胞和血管内皮细胞,IL-1β在腺泡腔中的白细胞中也有表达。由此可见,LPS处理大鼠乳腺后ICAM-1、TNF-α和IL-1β这三种细胞因子均参与了乳腺炎症过程中的免疫反应。Objective： This experiment was conducted to study the expression of ICAM-1,TNF-α and IL-1β during LPS-induced rat mastitis.Method： LPS infected mammary gland of SD rat on the 5th day of lactation and RMMVECs（Rat Mammary Microvascular Endothelial Cells） in vitro were prepared for immunohistochemistry analysis of ICAM-1,TNF-α and IL-1β.Result： A positive expression of ICAM-1,TNF-α and IL-1β in mammary gland epithelial cells and mammary gland endothelial cells was detected after LPS stimulating,IL-1β either expressed in acinar lumina.In mammary tissue experiment,expression of ICAM-1,TNF-α and IL-1β were strongly up-regulated at 6 h,then the level of ICAM-1 and IL-1β were increased,but the level of TNF-α was decreased.In RMMVECs experiment,expression of ICAM-1,TNF-α and IL-1β were increased gradually.Conclusion： The results indicated that ICAM-1,TNF-α and IL-1β involved in immune response in LPS-induced mastitis.

关 键 词：大鼠乳腺炎 黏附分子 肿瘤坏死因子 白细胞介素 

分 类 号：S852.31[农业科学—基础兽医学]