黄连木组培快繁育苗研究  

On rapid propagation for tissue culture seedlings of Pistacia chinensis

作　　者：何敬房[1] 苏淑钗[2] 冷平生[1] 姬谦龙[3] 于涌鲲[3] 刘文科[4] 

机构地区：[1]北京农学院园林学院,北京102206 [2]北京林业大学林学院,北京100083 [3]北京农学院组培中心,北京102206 [4]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《北京农学院学报》2011年第3期44-47,共4页Journal of Beijing University of Agriculture

基　　金：北京市自然科学基金项目(6072005);北京市创新人才建设计划项目(PXM2009-014207-076874);北京农学院组培中心资助项目

摘　　要：以黄连木的枝条作为外植体进行组织培养。结果表明:1/2 DKW+6-BA 1.0 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L作为茎段腋芽诱导的培养基,芽体长达4 cm;1/2 DKW+6-BA 4.0 mg/L+IBA 0.04 mg/L+NAA 0.02 mg/L为增殖培养基,增殖系数可达4.21;1/2 DKW+IBA 6.0 mg/L为生根培养基,生根率达到91.15%;移栽在草炭∶蛭石∶粗砂=3∶1∶2混合基质中,成活率高达92.11%以上。The stem-segments were used as explants in the tissue cultrue of Pistacia chinensis Bunge.Results indicated that：Medium of 1/2DKW＋6-BA 1.0 mg/L＋NAA 0.1 mg/L＋IBA 0.2 mg/L was the best medium for the induction of axillary bud,and the bud＇s length was up to 4cm;Multiplication coefficient was 4.21 on the medium of 1/2 DKW＋6-BA 4.0 mg/L＋ IBA 0.04 mg/L＋NAA0.02 mg/L IBA 0.1 mg/L;Rooting rate was up to 91.15% on the medium of 1/2DKW＋ IBA6.0 mg/L;The suitable transplantation martix was peat∶verimiculite∶coarse sand（3∶1∶2）,and survial rate of plant with root was above 92.11%.

关 键 词：黄连木 组织培养 快速繁殖 生根 

分 类 号：S664.9[农业科学—果树学]