miR-98前体转染卵巢癌细胞的效率检测及增殖抑制检测  

Transfection efficiency of miR-98 precursor and proliferation inhibitory effect on cell proliferation in ovarian cancer cell lines

作　　者：王甜甜[1] 霍冠华[1] 

机构地区：[1]滨州医学院组织学与胚胎学教研室,烟台市264003

出　　处：《滨州医学院学报》2011年第3期164-169,共6页Journal of Binzhou Medical University

摘　　要：目的 pre-hsa-miR-98转染卵巢癌SKOV3、HO8910和A2780细胞,观察转染率并研究转染后对卵巢癌细胞的增殖抑制作用,为卵巢癌新治疗方法研究提供实验依据。方法利用构建的pre-hsa-miR-98质粒HmiR和连接随机序列的质粒CmiR转染人卵巢癌细胞SKOV3、HO8910和A2780细胞,荧光显微镜观察和流式细胞仪计数计算转染率,MTT法检测转染对卵巢癌细胞的增殖抑制作用。结果 HmiR转染后24 h,荧光显微镜下观察,SKOV3、HO8910、A2780细胞的转染率分别为61.50%、68.59%、63.40%;流式细胞术计数,转染HmiR后SKOV3、HO8910、A2780细胞的转染率分别为57.93%、66.73%、58.59%。转染HmiR后,MTT法检测SKOV3、HO8910、A2780细胞的增殖抑制率随转染时间延长而增加,72 h达到49.54%、53.25%、46.09%。结论三种卵巢癌细胞的转染率均接近或超过60%,HmiR转染后可抑制卵巢癌细胞增殖,其抑制作用随转染时间延长而增强。Objective The pre-hsa-miR-98 was transfected into ovarian cancer SKOV3,HO8910 and A2780 cells,and to investigate the transfection efficiency and proliferation inhibition,in hopping of providing experimental evidence for treating ovarian malignances.Methods The pre-hsa-miR-98 plasmid HmiR and plasmid CmiR,from a control vector,were transfected into human ovarian cancer cell lines SKOV3,HO8910 and A2780.Transfection efficiency was investigated by fluorescence microscopy and flow cytometry,and the proliferation inhibition in the cell lines were tested by MTT assay.Results After transfected 24 hours,the rates of transfection in SKOV3,HO8910,A2780 cells by fluorescence microscopy were 61.50%,68.59%,and 63.40%.The transfection rates by flow cytometry were 57.93%,66.73%,58.59% respectively.The rates of proliferation inhibition in the cells after HmiR transfection measured by MTT assay went up with the increasing of the hours of transfection,and came up to 49.54%,53.25%,46.09% after transfection 72 hours.Conclusion The transfection rates of the three cell lines transfected by pre-hsa-miR-98 HmiR close to or over 60%,and the transfection inhibited the proliferation of cell lines in a time-dependent manner.

关 键 词：微小RNA98 卵巢癌细胞 转染率 增殖抑制 

分 类 号：R392.1[医药卫生—免疫学]