联合检测CEA、IFN-γ及ADA在结核性和恶性胸腔积液鉴别诊断中的价值  

Clinical study on the diagnostic value of CEA,interferon-γ and ADA in differentiating tuberculous pleural effusion from malignant pleural effusion

作　　者：吕鹏[1] 张良明[2] 耿冬梅[2] 刘云波[3] 刘丹萍[4] 

机构地区：[1]滨州医学院临床学院,滨州市256603 [2]烟台市毓璜顶医院肿瘤内科 [3]滨州医学院校医院 [4]烟台市毓璜顶医院神经内科

出　　处：《滨州医学院学报》2011年第3期189-191,共3页Journal of Binzhou Medical University

摘　　要：目的通过检测良恶性胸腔积液中癌胚抗原(carcinoembryonic antigen,CEA)、γ-干扰素(interferon-γ,IFN-γ)及腺苷酸脱氨酶(adenosine deaminase,ADA)的表达情况,探讨三者在结核性与恶性胸腔积液鉴别中的价值。方法选取70例胸腔积液患者,结核性胸腔积液32例,恶性胸腔积液38例,分别采用酶联免疫吸附实验(ELISA)及比色分析法检测胸腔积液中IFN-γ和CEA、ADA的表达。结果 CEA诊断恶性胸腔积液的敏感性、特异性及准确性分别为76.32%、87.50%及81.43%;ADA及INF-γ诊断结核性胸腔积液的敏感性、特异性及准确性分别为78.13%、81.58%、80.00%和90.63%、97.37%、94.29%。三者联合检测对恶性及结核性胸腔积液的敏感性、特异性及准确性分别为94.74%、96.88%、95.71%和93.75%、97.37%、95.71%。结论联合检测胸腔积液中CEA、IFN-γ和ADA更有助于结核性与恶性胸腔积液的鉴别诊断。Objective To study the diagnostic value of carcinoembryonic antigen（CEA）,interferon-γ（IFN-γ）and adenosine deaminase（ADA） in differentiating tuberculous and malignant pleural effusions by detecting their expression in the pleural effusions.Methods 70 cases of pleural effusion were selected,including tuberculous pleural effusion in 32 cases and malignant pleural effusion in 38 cases respectively,the expression of carcinoembryonic antigen,interferon-γ and adenosine deaminase in the pleural effusions was examined by using ELISA and colorimetric analysis.Results Diagnosis of malignant pleural effusion CEA sensitivity,specificity and accuracy were 76.32%,87.50% and 81.43%.ADA and INF-γ diagnosis of tuberculous pleural effusion,sensitivity,specificity and accuracy were 78.13%,81.58%,80.00% and 90.63%,97.37%,94.29%.Combined detection of malignant and tuberculous pleural effusion,sensitivity,specificity and accuracy were 94.74%,96.88%,95.71% and 93.75%,97.37%,95.71%.Conclusion Combine detection of CEA,IFN-γ and ADA can diagnosis tuberculous and malignant pleural effusions.

关 键 词：胸腔积液 癌胚抗原 Γ-干扰素 腺苷酸脱氨酶 酶联免疫吸附实验 

分 类 号：R446.1[医药卫生—诊断学]