七带石斑鱼精子活力及其与环境的关系  

Relation of environmental factor and spermatozoa vitality of Epinephelus septemfasciatus

作　　者：区又君[1] 廖光勇[1,2] 陈超[3] 李加儿[1] 庄志猛[3] 柳学周[1] 杨志 孙涛 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]中国水产科学研究院黄海水产研究所,山东青岛266071 [4]烟台开发区天源水产有限公司,山东烟台264003

出　　处：《海洋环境科学》2011年第4期516-519,共4页Marine Environmental Science

基　　金：国家"863"项目(2006AA10A414)

摘　　要：研究了不同盐度和温度下七带石斑鱼(Epinephelus septemfasciatus)精子的活力和低温保存下精子的寿命,以及七带石斑鱼精子的超低温保存。结果表明,七带石斑鱼精子活力最强时的最适盐度为30,最适温度为22.8,激烈运动时间最长为33.99 m in;精子涡动时间和总活动时间与盐度和温度的关系均为二次函数关系;15以下的低盐度水和淡水对七带石斑鱼精子活力具有可逆的抑制作用。精子在4下低温保存1周以上能保持较强活力。超低温保存精子解冻后的存活率为10%以上,其最适激活盐度为25。The effects of salinities and temperatures on the spermatozoa vitality, and cryopreservation of the spermatozoa of Epinephelus septemfasciatus were investigated. The results showed that the spermatozoa vitality was the highest under the condition of salinity 30 and water temperature of 22.8℃ ,with the longest eddying time of 33.99min. The quadratic function correlations were found between eddying time of spermatozoa and both salinity and temperature, as well as between total active time and both that. The motility of spermato zoa were inhibited reversibly in low-salinity （ 〈 15 ppt） and freshwater. The strong vitality of spermatozoa was kept for longer than one week preserved in low temperature below 4℃. The survival rate of spermatozoa eryopreservation was over 10% with the optimal activated salinity of 25.

关 键 词：七带石斑鱼 精子 活力 环境因子 

分 类 号：Q175[生物学—水生生物学]