大连近岸海域经济贝类麻痹性贝毒分析  

Analysis of paralytic shellfish poisoning in shellfish of Dalian coast

作　　者：宋普江 张伟[2] 王刚[3] 姬文婷[2] 李伟[2] 佟长青[2] 金桥[2] 

机构地区：[1]黑龙江粮油食品进出口公司,黑龙江哈尔滨150036 [2]辽宁省水产品加工与综合利用重点开放实验室,大连海洋大学食品工程学院,辽宁大连116023 [3]辽宁出入境检验检疫局,辽宁大连116000

出　　处：《海洋环境科学》2011年第4期533-535,545,共4页Marine Environmental Science

基　　金：辽宁省教育厅2009度高等学校科研项目计划(2009A163)

摘　　要：对2007年1月至2008年10月间大连地区近岸海域经济贝类的麻痹性贝毒采用小鼠生物法检测,结果表明:2007年4月至9月及2008年2月至8月贝类中的麻痹性贝毒超标情况较多,超标率较高的地点是长海财神岛、庄河黑岛。在染毒的贝类种类中,主要以虾夷扇贝为主,在杂色蛤和牡蛎中未检出麻痹性贝毒。通过ELISA的方法研究了虾夷扇贝不同组织中的蛋白质对麻痹性贝毒的结合能力。采用DEAE-52离子交换层析和Sephadex G-100层析方法分离出麻痹性贝毒结合蛋白,通过SDS-PAGE测定出麻痹性贝毒结合蛋白的分子量分别为113 kDa和72.4 kDa。The economic shellfish in Dalian coast were collected from Jan. 2007 to Oct. 2008. The paralytic shellfish poisoning（PSP） in shellfish was determined by the mouse bioassay. The results showed that the high values of PSP of economic shellfish were found from Apr. to Sep. of 2007 and from Feb. to Aug. of 2008 in Dalian coast. The high values of PSP were found in Calshen Island of Changhai and Hei Island of Zhuanghe. The major findings of PSP were in scallop Patinopecten yessoensis, not in clams Scrobicularia plana and oyster Crassostrea gigas. The paralytic shellfish poison-binding proteins （PSPBP） in different tissues were studied by ELISA and the result showed that the proteins in muscle strongly bind with PSP. PSPBP were isolated by DEAE 52 and Sephadex G-100. SDS-PAGE showed that the PSPBP has a molecular mass of 113 kDa and 72.4 kDa.

关 键 词：大连近岸 麻痹性贝毒 虾夷扇贝 麻痹性贝毒结合蛋白 

分 类 号：Q331[生物学—遗传学]