固体基质室温磷光法测定布美他尼与人体疾病预报  

Determination of Trace Bumetanide and Forecast of Human Diseases by Solid Substrate-room Temperature Phosphorimetry

作　　者：林璇[1] 

机构地区：[1]漳州师范学院化学与环境科学系,福建漳州363000

出　　处：《漳州师范学院学报（自然科学版）》2011年第2期39-44,共6页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：福建省教育厅科技项目(JA10203)

摘　　要：本研究根据布美他尼(BMTN)抑制Fe3+与桑色素(R)生成络合物[Fe-morin]3+([FeR]3+)而导致体系的室温磷光信号显著猝灭进行的.依据抑制[Fe-桑色素]3+络合物形成效应、BMTN含量与△Ip的线性关系、发展了一种新的测定BMTN的抑制络合反应固体基质室温磷光法(SS-RTP).本方法的检出限为5.0 ag spot-1(对应浓度1.2×10-14 g ml-1),线性范围为0.040-4.0 pg ml-1,显示了高的灵敏度与宽的线性范围,用于人体尿液中BMTN的测定,结果与UV相吻合.同时,用摩尔比法、等摩尔比连续变化法和红外光谱确定络合物的组成为[FeR]3+。A novel solid substrate-room temperature phosphorimetry（SS-RTP） was developed for determination of bumetanide（BMTN）.It was validated by determining selectivity,linearity,accuracy,precision,and signal to noise ratio（S/N） for analysis.And all the experiments presented in this work were based on that BMTN inhibited the formation of [Fe-morin]3＋（[FeR]3＋） complex by the reaction between Fe3＋ and R,which led to severe quenching of room temperature phosphorescence（RTP） signal.Detection limit of this method（LD,5.0 ag spot-1,corresponding concentration was 1.2 × 10-14 g mL-1） was evaluated and compared with other methods,indicating better sensitivity for BMTN determination using this technique.And due to the high sensitivity of the method,it has been successfully applied to determine BMTN in human urine samples.The linear range was from 0.040 to 4.0 pg mL-1,allowing wide determined range of BMTN.Meanwhile,the mechanism of this method was also discussed.

关 键 词：布美他尼 桑色素-Fe3+络合物 抑制效应 固体基质室温磷光法 人体疾病预报 

分 类 号：O629.8[理学—有机化学]