荧光定量PCR检测尖锐湿疣皮损Toll样受体的表达水平  

Expression of Toll-like receptors in the lesions of condyloma acuminatum

作　　者：朱小霞[1,2] 程浩[1] 张行[1] 朱可建[1] 周强[1] 陈大方[1] 王琦[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院,杭州310016 [2]宁波市第一医院,315000

出　　处：《中华微生物学和免疫学杂志》2011年第6期560-563,共4页Chinese Journal of Microbiology and Immunology

基　　金：浙江省卫生高层次创新人才培养计划;国家自然科学基金（81071302）

摘　　要：目的检测尖锐湿疣皮损中Toll样受体（TLRs）的mRNA表达水平，探讨TLRs在人乳头瘤病毒（HPV）感染免疫中的可能作用。方法提取40例尖锐湿疣患者皮损和35例HPV阴性的慢性宫颈炎患者宫颈刮落细胞的总RNA，逆转录为cDNA后用实时荧光定量PCR法检测TLRl～10的mRNA表达水平，并检测40例尖锐湿疣的HPV分型。结果40例尖锐湿疣患者以低危型HPV6和11型为主（77．5％和55％）。55％尖锐湿疣患者感染两种以上HPV亚型，其中35％合并高危型HPV感染。尖锐湿疣组TLR3、7、8的mRNA表达水平高于其他TLRs，TLR9的mRNA表达水平低于其他TLRs（P均〈0．05）。尖锐湿疣皮损TLR1—10的mRNA表达水平在HPV6型和HPV11型感染间以及在低危型与合并高危型HPV感染间差异无统计学意义（P均〉0．05）。尖锐湿疣组TLR1～3、TLR5～8和TLR10的mRNA表达水平均高于HPV阴性的慢性宫颈炎组（P均〈0．05），其中TLR2、7、8的mRNA表达水平升高更显著（P均〈0．01）。而TLR4、11LR9的mRNA表达水平与对照组差异无统计学意义（P〉0．05）。结论尖锐湿疣皮损中部分TLRs（3、7、8）表达水平较高，但TLR9表达水平较低。相对于HPV阴性的慢性宫颈炎，尖锐湿疣皮损中TLR1—3、TLR5～8和TLR10的mRNA表达上调。不同HPV型别感染的尖锐湿疣皮损中TLRs表达谱差异无统计学意义。推测尖锐湿疣TLRs表达谱的改变可能与HPV感染有一定关系，是否与HPV感染的免疫逃逸机制及持续感染有关尚有待进一步明确。Objective To investigate the expression of Toll-like receptors （TLRs） in condyloma acuminatum（CA） lesions and their possible roles in the pathogenesis of CA. Methods The expressions of TLRI-10 mRNA level in the lesions of CA and in the cervix scrape cells from the patients with human papil- lomavirus（HPV） negative chronic cervicitis were detected by real-time quantitative fluorescent PCR. HPV typing was detected by HPV GenoArray test kit. Results Low-risk HPV type 6 and type 11 were the most prevalent types in the forty CA cases with positive rate of 77.5% and 55% respectively. 55% CA patients were found infected with more than two types of HPV. 35% CA patients were concurrently infected with high-risk HPV. The expressions of TLR3, 7, 8 mRNA were higher than other TLRs and the expression of TLR9 mRNA was lower than others in the lesions of CA. No significant differences of the TLRI-10 mRNA levels were found between HPV6 and HPV11 positive CA lesions, so did it between low-risk and high-risk HPV concurrent infected CA lesions. The expressions of TLR1-3, TLRS-$, TLR10 mRNA, especially TLR2, TLR7 and TLR8 in the lesions of CA were significantly higher than that in cervix scrape cells of HPV negative chronic cervicitis. There were no significant differences of TLR4 and TLR9 mRNA levels between the two groups. Conclusion There were higher expressions of some TLRs （3, 7, 8） and lower expression of TLR9 in the lesions of CA. Compared with HPV negative chronic cervicitis, the expressions of TLR1-3, TLRS-S, TLR10 mRNA in the lesions of CA were up-regulated. The expression profile of TLRs in different type of HPV infected CA lesions had no significant differences. Our results suggested that the expression profile of TLRs in CA may be associated with the HPV infection. Whether it was associated with the immune es- cape mechanism and persistent infection of HPV need further demonstration.

关 键 词：荧光定量PCR 尖锐湿疣 人乳头瘤病毒 TOLL样受体 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]