罗格列酮对成纤维细胞条件培养液诱导心肌肥大的作用及机制  

Effects of rosiglitazone on fibroblast conditioned growth medium-induced myocardial hypertrophy and its mechanism in rats

作　　者：朱肖星[1] 徐琳[2] 陈定章[1] 啜玉彩[3] 唐发宽 徐云霞[5] 李华[5] 周晓东[1] 王文清[6] 朱妙章[7] 

机构地区：[1]第四军医大学西京医院超声科,西安710032 [2]广州军区广州总医院心内科,广州510010 [3]第四军医大学西京医院老年病科,西安710032 [4]解放军总参总医院心内科,北京100091 [5]第四军医大学校直干休所,西安710032 [6]空军总医院院涉外病房,北京100142 [7]第四军医大学基础部,西安710032

出　　处：《解放军医学杂志》2011年第7期721-724,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(30870902);中国博士后基金(20090461448)

摘　　要：目的探讨罗格列酮(RSG)对成纤维细胞无血清条件培养液(FCGM)诱导心肌肥大的作用及其机制。方法分离获取新生大鼠成纤维细胞,用含10g/L胰岛素+转铁蛋白+硒化钠复合物(ITS)的无血清高糖DMEM培养,分别于第3、5、79、天收集FCGM,ELISA法检测其中内皮素-1(ET-1)的浓度。采用FCGM培养新生大鼠心肌细胞,检测其蛋白含量和蛋白激酶C(PKC)活性,并观察白屈菜季氨碱(che)、RSG对FCGM诱导心肌蛋白质合成的影响。结果 FCGM中含有ET-1,且培养3d时的浓度最高。FCGM可促进心肌细胞蛋白质合成,而RSG和che可阻断FCGM促心肌细胞蛋白质合成的作用并抑制心肌细胞PKC的活性。结论 FCGM诱导心肌肥大与其中的ET-1有关,RSG抑制心肌肥大的作用与抑制ET-1和PKC的活性有关。Objective To investigate the inhibitory effect of rosiglitazone（RSG） on fibroblast conditioned growth medium（FCGM）-induced myocardial hypertrophy and its mechanism in rats.Methods FCGM-stimulated protein synthesis and protein kinase C（PKC） activity were measured in neonatal rat ventricular cardiomyocytes in the absence or presence of RSG or the PKC inhibitor chelerythrine（che）.Results Cultured neonatal rat ventricular fibroblast conditioned growth medium significantly enhanced protein synthesis of cardiomyocytes.Meanwhile ET-1 was detected in FCGM using the enzyme-linked immunosorbent assay（ELISA）.RSG and che counteracted the growth promoting effect of FCGM possibly via suppressing PKC activity.Conclusion FCGM-induced myocardial hypertrophy may be associated with ET-1.The inhibitory effects of RSG on myocardial hypertrophy may be mediated through ET-1 and PKC.

关 键 词：内皮缩血管肽-1 罗格列酮 蛋白激酶C 成纤维细胞 肌细胞 心脏 

分 类 号：R542.2[医药卫生—心血管疾病]