5-N甲-基化苯并呋喃[3,2-b]喹啉衍生物的合成及其与小牛胸腺DNA相互作用  

Synthesis of 5-N-methylated Benzofuro [3,2-b] Quinoline Derivatives and Interaction Between Derivatives and Calf Thymus DNA

作　　者：卢宇靖[1] 张瑞瑞[1] 杜志云[1] 方岩雄[1] 张焜[1] 

机构地区：[1]广东工业大学轻工化工学院

出　　处：《光谱实验室》2011年第4期1565-1569,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(21042003)

摘　　要：设计并合成了天然产物喹叨啉(Quindoline)的两个衍生物:苯并呋喃[3,2-b]喹啉和5-N-甲基苯并呋喃[3,2-b]喹啉,通过MS、1H NMR等手段确认其结构。运用紫外(可见光谱、荧光光谱和圆二色光谱研究了合成的衍生物与小牛胸腺DNA结合能力的强弱和结合方式。实验结果表明,5-N季铵化引入的正电荷能提高苯并呋喃喹啉衍生物与小牛胸腺DNA的结合能力;圆二色光谱表明合成的衍生物是以嵌插方式与小牛胸腺DNA相互作用。The two natural products quindoline derivatives,benzofuroquinoline and 5-N-methyl benzofuroquinoline,were synthesized,and their structures were characterized by MS and 1H NMR.The binding mode and binding ability of these derivatives with calf thymus DNA were studied by UV-Vis absorption spectra,fluorescence spectra and circular dichroism spectra.The introduction of a positive charge by N-methylation at 5-N position significantly improved the binding ability of derivatives and calf thymus DNA.Results from circular dichroism spectra revealed that the intercalative mode of these derivatives and calf thymus DNA.

关 键 词：苯并呋喃[3 2-b]喹啉 小牛胸腺DNA 荧光光谱 圆二色光谱 

分 类 号：O657.2[理学—分析化学] O657.6[理学—化学]