光谱法研究Leu-ENK与ct-DNA的相互作用  

Interaction of Leu-ENK with ct-DNA by Spectroscopy

作　　者：马丽[1] 姚程成[1] 卢奎[1,2] 牛乐朋[1] 

机构地区：[1]河南工业大学化学化工学院,郑州市高新技术开发区莲花街450001 [2]河南工程学院材料与化学学院,郑州市新郑龙湖中山北路1号451191

出　　处：《光谱实验室》2011年第4期1588-1592,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(20572016;20772023);河南省教育厅科技计划(2006KYCX017)

摘　　要：亮氨酸脑啡五肽是动物体内一种具有很强生物活性的阿片肽,在生物体内有极其重要的作用。利用紫外光谱和荧光光谱法研究了亮氨酸脑啡五肽(Leu-ENK)与小牛胸腺DNA(ct-DNA)的相互作用。研究表明,Leu-ENK-ct-DNA体系随着Leu-ENK浓度增大,体系的紫外光谱呈增色效应;随着ct-DNA浓度的增加,体系Leu-ENK的荧光逐渐猝灭;这两种方法的实验结果都表明两者的作用模式主要为嵌入结合,根据Stern-Volmer方程可知Leu-ENK-ct-DNA体系的作用属于静态猝灭。计算得到两者作用的结合常数达到2.54×104L/mol,结合位点数为1.014。Leucine enkephalin which is a strong activity of peptides has important effect in vivo.The interaction of Leu-ENK with ct-DNA was studied by UV spectrometry and fluorescence spectroscopy.The ultraviolet spectrum of Leu-ENK-ct-DNA system produced hyperchromicity effect with increasing the concentration of Leu-ENK.The fluorescence of Leu-ENK had quenching effects with increasing the concentration of ct-DNA,and it can be concluded that the interaction between Leu-ENK and ct-DNA was embed mode.The interaction of Leu-ENK-ct-DNA system was static quenching,according to Stern-Volmer equation.The binding constant was calculated to be the class of 2.54×10^4L/mol with numbers of binding sites of 1.014.

关 键 词：亮氨酸-脑啡五肽 紫外光谱法 荧光光谱法 CT-DNA 相互作用 

分 类 号：O657.32[理学—分析化学]