iTRAQ技术及其在蛋白质组学中的应用  

ITRAQ Technology and Its Application in Proteomics

作　　者：谢秀枝[1] 王欣[2] 刘丽华[1] 董世雷[3] 皮雄娥[2] 刘伟[2] 

机构地区：[1]内蒙古工业大学化工学院,呼和浩特010051 [2]浙江省农业科学院植物保护与微生物研究所,杭州310021 [3]浙江省师范大学化学与生命学院,金华321004

出　　处：《中国生物化学与分子生物学报》2011年第7期616-621,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国际科技合作项目(No.2008DFA32080)~~

摘　　要：近年来随着蛋白质组学的迅速发展,其相应的方法学研究也取得了巨大的进步,一系列新技术融入了蛋白质组学研究中,极大地促进了这门学科的发展.相对和绝对定量同位素标记(iTRAQ)技术与高度敏感性和准确性的串联质谱及多维液相色谱联用技术已成为蛋白质定性和定量研究的主要工具之一.该技术可对复杂样本、细胞器、细胞裂解液等样本进行相对和绝对定量研究,具有较好的定量效果、较高的重复性.由于其能够同时对多达8种样品进行标记分析,故在生命科学的各个领域得到了广泛的应用.本文对iTRAQ的原理、实验流程、优缺点及近几年的应用进展进行综述.In the past years,proteomics was developed quickly and its corresponding methodological research has also been made great progress,a series of new technologies blended in proteomics technology,particularly the new combination of multi-dimensional liquid chromatography and mass spectrometry（MS）-based technologies with protein fragmentation methods,enable us easily to identify the dynamics of proteins profiles in any complex biological process.Isobaric tags for relative and absolute quantification（iTRAQ） is one of the new techniques with high sensitivity and accuracy,demonstrating a remarkable advantage in simultaneous analysis of multiple samples and subsequently providing the relative quantification on hundreds of proteins at one time.The iTRAQ reagent produced high quality,reproducible result in enriched complexes,organelles,and whole cell lysates.Hence iTRAQ has been widely used in areas of life science.This review will focus on the principle,experimental procedure and application of iTRAQ in the last few years.

关 键 词：相对和绝对定量同位素标记(iTRAQ)技术 蛋白质组学 串联质谱 多维液相色谱 生物标记物 

分 类 号：Q51[生物学—生物化学]