水芹水浸提液对小球藻生长及抗氧化酶活性的影响  

Effects of Watercress Extracts on Growth and Activities of Antioxidant Enzymes of Chlorella vulgaris

作　　者：宰学明[1,2] 钦佩[2] 袁亚光[2] 吴向华[2] 周文宗[2] 

机构地区：[1]金陵科技学院园艺学院,江苏南京210038 [2]南京大学盐生植物试验室,江苏南京210093

出　　处：《安徽农业科学》2011年第19期11433-11435,共3页Journal of Anhui Agricultural Sciences

基　　金：林业公益性行业科研专项(200904001)

摘　　要：[目的]研究水芹水浸提液对小球藻生长及其抗氧化酶活性的影响。[方法]采用小球藻在不同浓度的水芹水浸提液中纯培养的方法,测定小球藻生长速率、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、·O2-产生速率及丙二醛(MDA)含量的变化。[结果]10g/L水芹水浸提液处理下,SOD和POD活性逐步上升,至试验结束时都维持在较高的水平,·O2-产生速率和MDA含量与对照间无显著差异;20～50g/L水芹水浸提液处理,藻细胞的SOD、POD活性、·O2-产生速率及MDA含量均呈先升高后降低的趋势,并且有浓度效应。[结论]水芹水浸提液对小球藻具有化感效应。10g/L水芹水浸提液对小球藻的生长具有明显的促进效应,20～50g/L水芹水浸提液对小球藻有明显的克生作用。[Objective]The effects of watercress extracts on the growth and activities of antioxidant enzymes of Chlorella vulgaris were studied.[Method]In pure culturing Chlorella vulgaris with different concentrations of watercress extract the growth,SOD and POD activity,·O2-generating rate and MDA content of Chlorella vulgaris were determined.[Result]Under the treatment of 10 g/L watercress extract the activity of SOD and POD increased gradually and maintained upper level in the whole process,·O2-generating rate and the content of MDA didn’t have significant differences compared with control.Under the treatment of 20-50 g/L watercress extracts,the activity of SOD and POD,·O2-generating rate and the content of MDA initially increased,then decreased,and intensified with time extension and concentration increase.[Conclusion]Watercress extracts had allelopathy on Chlorella vulgaris.On the whole,10 g/L watercress extracts promoted the growth of Chlorella vulgaris significantly,and 20-50 g/L watercress extracts had obvious inhibition.

关 键 词：水芹 水浸提液 小球藻 生长 抗氧化酶 

分 类 号：S645[农业科学—蔬菜学]