三江白猪脂肪型脂肪酸结合蛋白基因多态性及多态性片段的序列分析  

Polymorphism of Adipocyte Fatty Acid-binding Protein Gene of Sanjiang White Pigs and Sequencing of Polymorphic Fragments

作　　者：黄玉兰[1] 黄大鹏[2] 李祥辉[3] 

机构地区：[1]黑龙江八一农垦大学生命科技学院,黑龙江大庆163319 [2]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [3]黑龙江八一农垦大学高等职业技术学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2011年第7期791-793,801,共4页Chinese Journal of Biologicals

基　　金：大庆市科技局攻关项目(200232)

摘　　要：目的分析三江白猪脂肪型脂肪酸结合蛋白(Adipocyte fatty acid-binding protein,A-FABP)基因的多态性及其多态性片段序列。方法采用PCR-RFLP检测100头三江白猪A-FABP基因第1内含子区的遗传多态性,并对多态性片段进行序列分析。结果在Bsu36Ⅰ-RFLP和BsmⅠ-RFLP位点上均发现了多态性,基因型分别为DD、Dd、dd和Bb、bb型。等位基因D的频率为0.67,等位基因B的频率为0.21。在BsmⅠ-RFLP和Bsu36Ⅰ-RFLP位点上,多态信息量(Polypeptide information con-tent,PIC)分别为0.277和0.336,为中度多态(0.25<PIC<0.5)。BsmⅠ-RFLP和Bsu36Ⅰ-RFLP变异的酶切位点分别在5006和4624位,均发生了C到G的突变,测序结果与GenBank中登录的序列(X16039)的同源性为99.6%。结论在三江白猪A-FABP基因的BsmⅠ和Bsu36Ⅰ酶切位点上存在多态性,多态性片段的碱基发生了突变。Objective To analyze the polymorphism of adipocyte fatty acid-binding protein（A-FABP） gene of Sanjiang white pigs as well as the sequence of polymorphic fragments.Methods The intron 1 of A-FABP gene of 100 Sanjiang white pigs were analyzed for genetic polymorphism by PCR-RFLP method,and the polymorphic fragments were sequenced.Results Polymorphisms were observed in both Bsu36Ⅰ-RFLP（genotypes DD,Dd and dd） and BsmⅠ-RFLP（genotypes Bb and bb） sites.The frequencies of alleles D and B were 0.67 and 0.21 respectively.The polypeptide information contents（PICs） at BsmⅠ-RFLP and Bsu36Ⅰ-RFLP sites were 0.277 and 0.336 respectively,indicating a moderate polymorphism（0.25 〈 PIC 〈 0.5）.Both BsmⅠ-RFLP and Bsu36 Ⅰ-RFLP products showed mutations of C→G,at sites 5006 and 4624 respectively.The homology of gene sequence of amplified fragment to that reported in GenBank（X16039） was 99.6%.Conclusion Polymorphisms were observed in both BsmⅠand Bsu36Ⅰsites of A-FABP gene of Sanjiang white pigs,and mutations in bases of polymorphic fragments.

关 键 词：三江白猪 脂肪酸结合蛋白质类 PCR—RFLP 

分 类 号：S828.91[农业科学—畜牧学] Q78[农业科学—畜牧兽医]