丹参病程相关蛋白基因(SmPR-10)的生物信息学及表达模式分析  

Bioinformatics and Expression Pathogenesis-related Protein 10 Gene(SmPR-10) from Salvia miltiorrhiza Bunge

作　　者：生华 刘梅[1] 化文平[1] 王喆之[1] 

机构地区：[1]教育部药用植物资源与天然药物化学重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,西安710062

出　　处：《植物科学学报》2011年第3期340-346,共7页Plant Science Journal

基　　金：国家自然科学基金资助项目(31000134);陕西省科技研究发展(攻关)计划资助项目(2006K16-G15)

摘　　要：对丹参EST序列进行Blast分析,获得了一条病程相关蛋白10(Pathogenesis-related protein10,PR-10)基因,命名为:SmPR-10(GenBank注册号:HM439764)。分别从cDNA和gDNA水平克隆得到该基因,其序列无内含子,包含一个长为486bp的完整开放读码框,编码161个氨基酸残基。生物信息学分析显示,SmPR-10所编码的蛋白SmPR-10具有Betv1结构域,属于病程相关蛋白10家族,其相对分子量为17·47kD,为稳定的酸蛋白;无信号肽、膜锚定或跨膜结构域,为亲水性蛋白。实时定量PCR结果分析表明,SmPR-10基因主要在丹参茎中表达,其表达受到病原菌和茉莉酸甲酯逆境信号的诱导,显示SmPR-10基因可能在植物防御反应中发挥作用。After analysis of Salvia miltiorrhiza EST sequences,one sequence was found had high homology with pathogenesis-related protein 10.The cDNA and DNA sequence of the gene were cloned and named as SmPR-10（GenBank accession number：HM439764）.SmPR-10 without intron contained a single opening reading frame of 486 bp encoding 161 amino acid-peptides.SmPR-10 had a Bet v1 domain and was classed into the pathogenesis-related protein 10 family according to its sequence.Bioinformatics showed that the putative protein was a stable acidic protein with a predicted molecular weight of 17.47 kD.There were no signal peptides and transmembrane domains in SmPR-10.The Quantitative RT-PCR results revealed that SmPR-10 was expressed in different organs of S.miltiorrhiza,and could be induced by methyl jasmonate and pathogen.This suggested that SmPR-10 may be involved in plant defenses.

关 键 词：SmPR-10 生物信息学分析 表达模式 

分 类 号：Q945.7[生物学—植物学]