焦核龙眼种子败育的cDNA-AFLP分析  

cDNA-AFLP Analysis on Seed Abortion in Longan(Dimocarpus longan Lour.)

作　　者：唐晰[1,2] 谢东丽[2] 刘潇[2] 蒋际谋[3] 梁文裕[2] 陈伟[1,2] 

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福州350002 [2]福建农林大学生命科学学院,福州350002 [3]福建省农业科学院果树研究所,福州350013

出　　处：《植物科学学报》2011年第3期347-351,共5页Plant Science Journal

基　　金：国家自然科学基金(30370999);福建省自然科学基金(2009J01084);教育部博士点基金(200803890009)

摘　　要：采用cDNA-AFLP技术对龙眼(Dimocarpus longan Lour.)正常和败育的种子进行了差异表达分析,并对差异明显的片段进行回收、克隆、测序,以及利用NCBI中的BLAST数据库已知序列进行同源性比对。结果表明:在11条重复性较好的差异片段中有3个Transcript-derived fragments(TDFs)分别与Ca2+-ATP酶、纤维素酶、质体ATP/ADP转运蛋白具有较高的相似性,其中TDF2和TDF7下调表达,TDF5上调表达,这可能影响了龙眼种子发育过程中的许多重要代谢途径,从而引起龙眼种子败育;有4个TDFs与一些hypothetical蛋白的序列相似;另有4个TDFs在蛋白数据库中未找到匹配的序列,核酸数据库中比对的结果也显示较高的E-值,说明为非同源基因。cDNA-AFLP technology was applied to identify differentially expressed genes between normal and abortive longan（Dimocarpus longan Lour.） seeds.Transcript-derived fragments（TDFs） identified as differentially expressed genes were eluted,cloned,sequenced and compared with the database using BLAST at NCBI.The results showed that among 11 sequenced fragments,three TDFs presented similarity with Ca2＋-ATPase,cellulase,and plastidic ATP/ADP transporter,respectively,whose up-regulation or down-regulation expression might influence several important metabolic pathways in developing seeds and led to seed abortion.Four TDFs were similar with hypothetical protein,while the other four TDFs did not match with known proteins in the protein database,while sequence alignment from the DNA database also showed higher E-values,indicating they were non-homologous genes.

关 键 词：龙眼 种子败育 CDNA-AFLP TDF 

分 类 号：S188[农业科学—农业基础科学] Q949.747.1[生物学—植物学]