氧化型低密度脂蛋白对培养的内皮细胞一氧化氮合成和释放的影响  

The Effect of Oxidized Low Density Lipoprotein on Releasing Nitric Oxide from Cultured Endothelial Cells

作　　者：樊燕蓉[1] 刘虹[1] 薛龙增[1] 卢是月[1] 施蓉芬 姚汝琳[2] 

机构地区：[1]海军医学高等专科学校,南京210099 [2]山西医科大学,太原030001

出　　处：《中国动脉硬化杂志》1999年第4期345-347,共3页Chinese Journal of Arteriosclerosis

摘　　要：为观察氧化型低密度脂蛋白对内皮细胞一氧化氮生成的影响，采用体外细胞培养方法将含不同浓度氧化型低密度脂蛋白的ＤＭＥＭ 培养液（ 氧化型低密度脂蛋白终浓度分别为０ ｍｇＬ、５０ ｍｇＬ 、１００ ｍｇＬ 、２００ ｍｇＬ），与内皮细胞共同孵育（３７ ℃、５％ ＣＯ２）２４ ｈ 后，分别测上清液中乳酸脱氢酶活性和一氧化氮含量及细胞中一氧化氮合酶活性，并用细胞化学染色法记数各组细胞一氧化氮合酶阳性染色细胞数。结果发现，随着培养液中氧化型低密度脂蛋白浓度升高，上清液中乳酸脱氢酶活性增加和一氧化氮含量下降，细胞中一氧化氮合酶活性和一氧化氮合酶阳性染色细胞数下降，可见，氧化型低密度脂蛋白可损伤血管内皮细胞，降低细胞中一氧化氮合酶活性，抑制一氧化氮产生，这可能是氧化型低密度脂蛋白致动脉粥样硬化的原因之一。Aim This study was to seek the effect of oxidized low density lipoprotein(ox-LDL) on the activity of nitric oxide synthase(NOS)and releasing nitric oxide(NO)from culture endothelial cells in vitro. Methods The endothelial cells were exposed to different doses of ox-LDL from 0 to 200 mg/L and incubated (37℃、5% CO 2)for 24 hours, and the activity of LDH and NO amounts in the supernatant and the activity of NOS in endothelial cells were measured, and NOS positive staining cells were stained using NADPH-diaphorase (NADPH-d) histochemical method . Results Ox-LDL could increase the activity of LDH and decrease the NO amounts in the supernatant ,and the activity of NOS and numbers of NOS positive staining cell decrease in a dose effect manner. Conclusion All those suggested that ox-LDL could directly damage endothelial cell, and decrease the activity of NOS of endothelial cell, and NO releasing from endothelial cell. This may be one of mechanims that ox-LDL bring atherosclerosis.

关 键 词：低密度脂蛋白 内皮细胞 一氧化氮 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]