大鼠气管上皮耐受热缺血时限的研究  

Length of warm ischemic tolerance for epithelial regeneration in heterotopic rat tracheal isografts

作　　者：韩敬泉[1] 张凯[1] 崔键[1] 刘成[1] 赵桂彬[1] 辛衍忠[1] 郭庆凤[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,哈尔滨150001

出　　处：《中华器官移植杂志》2011年第7期430-432,共3页Chinese Journal of Organ Transplantation

基　　金：哈尔滨市优秀学科带头人基金（2011RFXYS077）

摘　　要：目的探讨肺移植无心跳供者气管上皮耐受热缺血的时限。方法将SD大鼠分为4组。对照组在大鼠有心跳的情况下取出气管，热缺血30min组、45min组和60min组分别在处死大鼠后30、45和60min时取出气管，然后将供者气管固定后分别植入相应受者的大网膜内。14d后，取出气管，进行病理学检查，并对上皮再生情况进行评分。结果热缺血30min组和对照组比较，无论是上皮厚度还是黏膜上皮细胞层数的差异均无统计学意义（P〉0．05）；虽然45rain组与对照组相比，上皮厚度的差异有统计学意义（P〈0．05），但45min组的气管上皮有正常黏膜结构，并且与对照组相比，上皮细胞层数差异无统计学意义（P〈O．05）；而热缺血60rain组，看不到正常的上皮结构，上皮也仅由1～2层黏膜上皮细胞覆盖。结论气管耐受热缺血的时限可能为45min。Objective To determine the length of warm ischemic （WI） tolerance in bronchial graft from non-heart-beating donors. Methods Forty-eight rats were randomly divided into 4 groups （each group having 12 rats） according to different WI durations including WI-0 rain （group A）, WI-30 min （group B）, WI-45 rain （group C） and WI-60 rain （group D）. In each group, the tracheae from 6 rats were respectively imbedded in greater omentum of other 6 rats, and 14 days later, the transplanted tracheae were taken from recipients to evaluate epithelial thickness and regeneration. Results Epithelial thickness and the degree of epithelial regeneration had no significant difference （P〉0. 05） between the syngeneic control group and the WI-30 minutes group. All of the grafts with WI duration of 45 rain were viable, but the epithelium was significantly thinner than that in the syngeneic control group （P〈0. 05）. However all of the grafts with WI duration of 60 rain showed lower viability rate. Conclusion The time limits of tolerance to WI of tracheal grafts from NHBDs may be 45 rain.

关 键 词：大鼠 气管 热缺血 上皮再生 

分 类 号：R655.3[医药卫生—外科学]