松梢象对红松萜烯类挥发物的触角电位及行为反应  

Electroantennogram and behavioral responses of Pissodes nitidus to terpene volatiles of Pinus koraiensis

作　　者：王琪[1] 彭璐[1] 严善春[1] 廖月枝[1] 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室

出　　处：《北京林业大学学报》2011年第4期91-95,共5页Journal of Beijing Forestry University

基　　金：黑龙江省科技攻关项目(GB06B304-4);黑龙江省杰出青年科学基金资助项目(JC200802)

摘　　要：本文研究了松梢象对寄主红松挥发物组分S型α--蒎烯、R型α--蒎烯、β--蒎烯、β--月桂烯、罗勒烯、莰烯、3--蒈烯及反--柠檬烯8种挥发物,在0.0004、0.004、0.04、0.4及0.8mol/L浓度下的触角电位反应及行为反应。结果表明:这8种挥发物单体在各浓度下均能引起松梢象的触角电位(EAG)反应,且相同挥发物EAG反应值随浓度的增加而增加;松梢象尤其对0.4和0.8mol/L浓度下的S型α--蒎烯、R型α--蒎烯反应较强。"Y"型嗅觉仪行为测定表明,除β--月桂烯无明显定向作用、反--柠檬烯具有明显的驱避作用外,其他6种挥发物对松梢象均表现出不同程度的诱引作用。反--柠檬烯在0.4和0.8mol/L下具明显驱避作用,驱避率达62.5%～80%;0.4mol/L的S型α--蒎烯、R型α--蒎烯和罗勒烯对松梢象诱引作用极显著,诱引率达80%以上。说明以上红松挥发物,尤其是S型α--蒎烯、R型α--蒎烯、罗勒烯及反--柠檬烯对松梢象具有较强的潜在行为调控作用。In this study, we tested the electroantennographic （EAG） and behavioral responses of the weevil （Pissodes nitidus） to eight volatile compositions released from red pine （Pinus koraiensis）. The volatiles include （ 1 S）-（ - ） -α-pinene, （ 1R ）- （ ＋ ） -α-pinene, （ 1S）-（ - ） -β-pinene, camphene, β-myrcene, careen, D-limonene and ocimene in a series of concentrations, i. e. , 0. 000 d, 0. 004, 0.04, 0.4 and 0.8 mol/L. The results show that the weevil had higher EAG responses to all eight volatiles in five concentrations compared with the control （P 〈 0.05） and its EAG responses intensified with increased concentrations of volatiles. （IS）-（ - ）-α-pinene and （1R）-（ ＋ ）-α-pinene caused the most significant EAG responses with concentrations of 0.4 and 0. 8 mol/L. The behavioral responses to the eight volatiles were also examined with a Y-tube olfactometer. The weevils presented significantly repellent behavior to D-limone with concentrations of 0.4 and 0.8 mol/L and the repellent rate was 62.5% to 80%. Except β-myreene, the weevils were attracted to the other six volatiles and most significantly inclined to 0.4 mol/ L of （1S）-（ -）-α-pinene, （IR）-（ ＋ ）-α-pinene and ocimene, with trapping rates of above 80%. These volatiles assayed in this study, especially （1S）-（ - ）-α-pinene, （1R）-（ ＋ ）-α-pinene, ocimene and D-limone, may be potentially useful as repellents or attractants for the control and management of P. nitidus.

关 键 词：松梢象 红松 挥发物 触角电位 行为反应 

分 类 号：S763.38[农业科学—森林保护学]