应用PCR技术对孕妇HCMV感染的前瞻性研究  

Prospective study on the HCMV infection in pregnant women by PCR.

作　　者：欧知硕[1] 宋文刚[1] 殷宪敏[1] 高丽英[1] 高广燕 

机构地区：[1]泰山医学院儿科教研室医学遗传室,泰安271000

出　　处：《中国优生与遗传杂志》1999年第6期30-31,共2页Chinese Journal of Birth Health & Heredity

基　　金：山东省卫生厅青年基金

摘　　要：应用聚合酶链反应（ＰＣＲ）技术对１５６ 例孕妇尿中ＨＣＭＶ 进行检测，并追踪观察１１４ 例新生儿脐血中ＨＣＭＶ 垂直传播情况。结果表明：１１ 例孕妇尿ＨＣＭＶＤＮＡ 检测阳性，孕期感染率为７－０５％ ，有异常妊娠史孕妇感染率（１４－７１ ％ ）较正常孕妇（４－９２ ％） 明显升高。９ 例ＨＣＭＶ 阳性孕妇有４ 例观察新生儿脐血ＨＣＭＶＤＮＡ 阳性，占４４％ ，而ＨＣＭＶＤＮＡ阴性孕妇无１ 例新生儿脐血出现阳性结果。我们认为ＰＣＲ方法是检测ＨＣＭＶ宫内感染的可靠而灵敏的指标，对ＨＣＭＶＤＮＡ阳性的孕妇应进一步检查羊水，如羊水ＨＣＭＶＤＮＡ也阳性，应终止妊娠。HCMV in the urine from 156 pregnant women was determined by PCR and a tracing study on the vertical transmission of HCMV in cord bood was made on 114 new-borns. Results were as following. HCMV-DNA in the urine from 11 pregnant women was pastive and the infection rate was 7.05%; the infection rate of pregnant women with a history of abnormal pregnancy was 14,71%, which was much highter than that of normal pregrant women; among 9 pregnant women with positive HCMV, 4 had positive HCMV-DNA in cord blood of new-borns(44%)while there was no positive HCMV-DNA in cord blood of new-borns in pregnant women with negative HCMV.We think that PCR is a quick and reliable index in detecting intranterine HCMV infection. The pregnant women with positive HCMV-DNA should also have amniotie fluid examined. If HCMV-DNA in the amniotic fluid is also positive, the pregnancy should be terminated.

关 键 词：孕妇 聚合酶链反应 巨细胞病毒感染 

分 类 号：R714.251[医药卫生—妇产科学] R512.990.4[医药卫生—临床医学]