缺氧时一氧化氮、血小板活化因子释放与多形核白细胞与肺微血管内皮细胞粘附有关  

THE RELEASE OF NITRO OXIDE AND PLATELET ACTIVE FACTOR ARE RELATED TO POLYMORPHONUCLEAR LEUKOCYTES ADHERENCE TO PULMONARY MICROVASCULAR ENDOTHELIAL CELLS DURING HYPOXIA

作　　者：黄瑊[1] 陈剑鸿[1] 罗德成[2] 

机构地区：[1]第三军医大学,400038 [2]重庆第三军医大学西南医院药局

出　　处：《高原医学杂志》1999年第3期18-20,共3页Journal of High Altitude Medicine

摘　　要：目的：本文旨在了解缺氧时多形核白细胞（ＰＭＮ）与肺微血管内皮细胞（ＰＭＶＥＣ）粘附对血管壁通透性影响的机理。方法：利用兔ＰＭＮ悬液与ＰＭＶＥＣ单层共育模型，观察缺氧时兔ＰＭＮ与ＰＭＶＥＣ粘附率、血小板活化因子（ＰＡＦ）、一氧化氮的变化（ＮＯ），通过ＣＤ１８ 单抗封闭试验观察了粘附率与ＰＡＦ、ＮＯ释放间的相关关系。结果与结论：缺氧时致的ＰＭＮ－ ＰＭＶＥＣ粘附明显影响了ＰＡＦ、ＮＯ的释放。To investigate why polymorphoneuclear leukocyte (PMN) adhesion to pulmonary microvasclular endothelial cell (PMVEC) can change vascular permeability during hypoxia Method:The cell culture platelets with PMN and/or cultured PMVEC were exposed to hypoxia (PO 2=15～20mmHg).And some of them were added monoclone antibody CD18 to change the adhesion.The adhesion rates and the NO and PAF were assayed.And the relation between ashesion rate and NO or PAF was analysised.Results:Comparing with normoxia,hypoxia increased the adhesion rates.The monoclone antibody reduced the adhesion rates,PAF and NO.And the adhesion rate was correlative to PAF or No.Conclusion:The release of NO and PAF are related to PMN adherence to PMVEC during hypoxia.

关 键 词：缺氧 PAF 一氧化氮 多形核白细胞 

分 类 号：R594.302[医药卫生—内科学] R364.4[医药卫生—临床医学]