十溴联苯醚BDE-209对人卵巢癌细胞及仓鼠卵巢上皮细胞生长的影响  

The influence of Deca-BDE on OVCAR-3 cells and CHO cells proliferation and cell cycle in vitro.

作　　者：刘晓燕[1] 李志华[1] 陈敦金[1] 

机构地区：[1]广州医学院第三附属医院妇产科广州市妇产科研究所,广州510150

出　　处：《现代妇产科进展》2011年第6期445-448,453,共5页Progress in Obstetrics and Gynecology

基　　金：广东省自然科学基金(No:915180200300000001);广州市教育局团队基金项目[(09年)环境(PBDE)与遗传优生(No:B94119)]

摘　　要：目的:探讨十溴联苯醚(BDE-209)对人卵巢癌细胞株OVCAR-3及中国仓鼠卵巢上皮细胞株CHO细胞增殖和细胞周期的影响。方法:采用MTT检测法、免疫荧光化学检测法以及流式细胞仪等方法观察不同浓度BDE-209(0～100nmol/L)作用于OVCAR-3和CHO细胞0～72h,其细胞形态学变化、OD值改变、K i67表达变化及细胞周期的变化。结果:(1)0～100nmol/L BDE-209作用于细胞72h,倒置显微镜下观察随着浓度增高细胞增殖明显,细胞间隙变小甚至消失,细胞密集排布,细胞形态变小;(2)MTT法检测发现BDE-209促进两种细胞增殖,其增殖率呈时间依赖性和浓度依赖性;(3)0～100nmol/L BDE-209作用72h后,K i67阳性细胞数量增多,表达强度增强,呈现浓度依赖性;(4)流式细胞术检测发现BDE-209改变了细胞周期分布,OVCAR-3细胞G2/M期及CHO细胞S期细胞比例明显增高,且增殖指数(PI)随药物浓度增加而升高。结论:BDE-209明显促进OVCAR-3细胞和CHO细胞体外增殖,并使G2/M期或S期细胞比例增加。Objective：To investage the influence of BDE-209 on OVCAR-3 cells and CHO cells proliferation and cell cycle in vitro.Methods：Using MTT,immunofluorescence（IMF） and Flow Cytometry（FACS）,we observed the cell morphology,OD value change,Ki67 expression and cell cycle disposition after different BDE-209（0~100nmol/L） treatment for 0~72h in OVCAR-3 cells and CHO cells.Results：（1）We observed that OVCAR-3 cell and CHO cell grew more rapidly and became closer together,and the cell space appeared to narrow.（2）Absorbance（A） of OVCAR-3 cells and CHO cells increased obviously after 0~100nmol/L BDE-209 treatment in dose-dependent and time-dependent manner as measured by MTT.（3）The number of Ki67-positive cells increased,and the density of Ki67 expression was stronger than that of NG after 0~100nmol/L BDE-209 treatment in dose-dependent manner.（4） BDE-209 could change OVCAR-3 and CHO cell cycle disposition with G2/M stage percentage of OVCAR-3 and S stage percentage of CHO cell increased,as well as proliferation index（PI）（P0.05）.Conclusion：BDE-209 can obviously promote OVCAR-3 cells and CHO cell proliferation in vitro,and can induce the ratio of G2/M or S increase.

关 键 词：十溴联苯醚 OVCAR-3 CHO MTT KI67抗原 细胞增殖 细胞周期 

分 类 号：R711.75[医药卫生—妇产科学]