细胞基质蛋白Cyr61和CTGF在子痫前期发病中的作用  

Roles of matricellular protein Cyr61 and CTGF in preeclampsia.

作　　者：陈茜[1] 刘燕燕[1] 徐晓燕[1] 陈汉平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《现代妇产科进展》2011年第6期473-476,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:检测细胞基质蛋白Cyr61和CTGF在重度子痫前期患者胎盘组织中的定位及表达,研究它们在子痫前期发病中的作用。方法:采用免疫组化SP法和实时荧光定量聚合酶链式反应(quantitative real time polymerase chain reaction,real-time PCR)方法检测胎盘组织中Cyr61和CTGF的表达,其中重度子痫前期患者34例,正常妊娠16例。结果:(1)细胞基质蛋白Cyr61和CTGF在正常妊娠孕妇及重度子痫前期患者胎盘组织中均有表达;(2)Cyr61在重度子痫前期患者胎盘组织的表达水平低于正常妊娠孕妇胎盘(P<0.01);(3)CTGF在重度子痫前期患者胎盘组织的表达水平高于正常妊娠孕妇胎盘(P<0.05);(4)Cyr61和CTGF mRNA在重度子痫前期患者胎盘组织的表达水平呈负相关(P<0.01)。结论:Cyr61表达下调和CTGF过度表达与子痫前期的发病有关,并在该病的发病过程中起到一定的作用。Objective：To detect locations and expression levels of Cyr61（cysteine-rich 61,Cyr61） and CTGF（connective tissue growth factor,CTGF） in the placentas of severe preeclampsia and explore their relations with preeclampsia.Methods：The expressions of matricellular proteins Cyr61 and CTGF in human placenta during normal pregnancy（n=16） compared with severe preeclamptic placentae（n=34） using immunocytochemistry and quantitative real time polymerase chain reaction.Results：（1）Cyr61 and CTGF were expressed in placentas of severe preeclampsia and normal pregnancy.（2）The expression of Cyr61 was down-regulated in severe preeclampsia compared with normal group（P0.01）.（3）The expression of CTGF was higher in severe preeclampsia than in normal pregnancy（P0.05）.（4）Transcriptional levels of Cyr61 and CTGF were negative correlation in the placentas from severe preeclampsia（P0.01）.Conclusion：The down-regulation of Cyr61 and excessive expression of CTGF may be related with preeclampsia and play a role in the pathophysiological process of preeclampsia.

关 键 词：子痫前期 基质蛋白 半胱氨酸蛋白61 结缔组织生长因子 

分 类 号：R714.245[医药卫生—妇产科学]