2型糖尿病患者抵抗素基因420C/G多态性与非酒精性脂肪肝的关系  

Relationship between nonalcoholic fatty liver disease and the-420 C/G single nucleotide polymorphism of resistin in type 2 diabetes mellitus patients

作　　者：张凌云[1,2] 赵家军[1] 金勇君[2] 李建周[2] 

机构地区：[1]山东大学附属省立医院内分泌科,济南250021 [2]滨州医学院附属医院内分泌科,山东滨州256603

出　　处：《山东大学学报（医学版）》2011年第7期1-5,共5页Journal of Shandong University:Health Sciences

摘　　要：目的研究2型糖尿病(T2DM)患者中抵抗素基因5'末端启动子区-420 C/G多态性与非酒精性脂肪肝(NAFLD)的相关性。方法选取2型糖尿病患者268例,分为非酒精性脂肪肝组156例,无脂肪肝组112例,正常对照组115例,采用聚合酶链反应-限制性片断多态性(PCR-RFLP)方法分析抵抗素420 C/G多态性基因型。结果抵抗素-420C/G位点单核苷酸多态性分布在2型糖尿病合并非酒精性脂肪肝组与正常对照组之间差异有统计学意义(P<0.05),在2型糖尿病患者中GG基因型发生非酒精性脂肪肝的风险高,是CC基因型的2.40倍(P<0.05),是C等位基因携带者(CC+CG)的2.08倍(P<0.05),GG基因型是2型糖尿病合并非酒精性脂肪肝患病的危险因素(OR=3.05,95%CI 1.19-7.86,P<0.05)。结论抵抗素基因420位点GG纯合子增加了2型糖尿病合并非酒精性脂肪肝的风险。Objective To investigate the relationships between the resistin gene 5,promoter region-420 C/G polymorphism and nonalcoholic fatty liver disease（NAFLD） in type 2 diabetes mellitus（T2DM）.Methods 268 T2DM were selected,including 156 NAFLD,112 without fatty liver disease,and 115 controls,to analyze the resistin 420 C/G polymorphism genotype by polymerase chain reaction-restriction fragment polymorphism.Result The distribution of single nucleotide polymorphism at the-420 site in the resistin gene in patients with concurrent T2DM and NAFLD was significantly different from the control（P〈0.05）.In T2DM,the GG genotype was more associated with a 2.40-fold increased risk for NAFLD than the CC genotype（P〈0.05） and 2.08-fold increased risk than the CC＋CG genotype（P〈0.05）.The GG genotype was a risk factor for the development of NAFLD in T2DM（OR=3.05,95%CI 1.19-7.86,P〈0.05）.Conclusion The GG genotype of resistin at-420 may increase the risk of NAFLD in T2DM.

关 键 词：2型糖尿病 抵抗素基因 单核苷酸多态性 非酒精性脂肪肝 

分 类 号：R587.1[医药卫生—内分泌]