盐酸氨基乙酰丙酸搽剂对实验性兔耳痤疮的疗效观察  

Effect of aminolevulinic acid hydrochloride on the acne model of rabbits′ ears

作　　者：张成梅[1] 武传涛[1] 尹志圣[1] 李春令[2,3] 于学慧[1] 王立[1] 刘兆平[1] 

机构地区：[1]山东大学实验动物中心,济南250012 [2]山东大学 [3]山东省医疗器械产品质量检验中心,济南250101

出　　处：《山东大学学报（医学版）》2011年第7期90-93,104,共5页Journal of Shandong University:Health Sciences

摘　　要：目的研究盐酸氨基乙酰丙酸(ALA)搽剂对新西兰家兔耳实验性痤疮的治疗作用。方法 32只新西兰家兔,于每只兔右耳内侧面耳管处2 cm×2 cm范围备皮,备皮处涂抹丙酸杆菌/油酸,建立痤疮模型,成模后随机分为5组。模型对照组:病变部位涂抹溶剂后模拟光动力(PDT)治疗,1次/周,连用3周;克林霉素甲硝唑组:患部涂克林霉素甲硝唑搽剂,3次/d,连用2周;维A酸霜组:患部涂维A酸霜,3次/d,连用2周;ALA-PDT单次治疗组:第1周,1次;ALA-PDF多次治疗组:1次/周,连用3周;正常对照组。ALA给药组及模型对照组每次给药后避光3 h,避光结束后使用ALA-PDT治疗,15 min/次。治疗结束后,取治疗处组织进行活检、组织切片、HE染色、显微镜观察及组织学判定。结果统计结果和组织切片显示,ALA-PDT对痤疮丙酸杆菌和油酸共同引起的家兔耳廓实验性痤疮有较好的治疗作用(P<0.01或P<0.05),克林霉素甲硝唑及维A酸霜治疗作用也比较明显(P<0.05),并且ALA-PDT多次治疗作用要强于ALA-PDT单次治疗及克林霉素甲硝唑和维A酸霜的治疗。结论 ALA-PDT多次治疗对痤疮丙酸杆菌/油酸共同引起的家兔耳痤疮有较好的治疗作用。Objective To investigate the therapeutic effect of aminolevulinic acid（ALA） linimentum on exprimental acne of New Zealand rabbits′ ears.Methods Thirty-two healthy adult New Zealand rabbits were used in this experiment.Propionibacterium acnes and oleic acid were administrated to the right inner ears（2 cm×2 cm range） of the rabbits to establish acne models.Then all the animals were randomly divided into 5 groups,① the model control group： photodynamics therapy（PDT） was given after solvent was applied in the disease region,once per week for 3 weeks,② the clindamycin metronidazole acne positive control group： clindamycin metronidazole was smeared in the disease region,3 times/d for 2 weeks,③ the retinoid positive control group： retinoid was applied in the disease region,3 times/d for 2 weeks,④ the ALA single treatment group： ALA was applied in the disease region,once in the first week,⑤ the ALA repeated treatment group： ALA was applied in the disease region,once per week for 3 weeks,and ⑥the normal control group.The animals in the ALA treatment groups and model control group were protected from light for 3 hours after administration,and then ALA photodynamic therapy（ALA-PDT） was given for 15 min/time.After treatment biopsy,and HE staining,microscopic and histological examination were carried out.Results Statistical results and biopsy showed that there was a good therapeutic effect on the rabbits′ acne models caused by propionibacterium acnes / oleic acid in the ALA-PDT treatment group（P0.01 or P0.05）.The effect in the other two positive treatment groups was also obvious（P0.05）,and the therapeutic effect in the ALA-PDT repeated treatment group was stronger than in the ALA-PDT single treatment group and the two positive drug groups.Conclusion Repeated ALA-PDT treatment has good therapeutic effect on rabbits′ acne models caused by propionibacterium acnes/oleic acid.

关 键 词：盐酸氨基乙酰丙酸 痤疮 模型 动物 光动力疗法 疗效 兔 

分 类 号：R965.3[医药卫生—药理学] R332[医药卫生—药学]