载Fe3O4全氟溴辛烷纳米粒增强超声显像的实验研究  

Fe3O4-loaded lipid perfluorooctylbromide nanoparticles as ultrasound contrast agents

作　　者：李奥[1] 王志刚[1] 左国庆[1] 余进洪[1] 郑元义[1] 

机构地区：[1]重庆医科大学超声影像学研究所重庆医科大学附属第二医院超声科, 400010

出　　处：《中华超声影像学杂志》2011年第7期625-627,共3页Chinese Journal of Ultrasonography

基　　金：基金项目：国家自然科学基金面上项目（30770566）

摘　　要：目的 探讨载磁性纳米材料Fe3O4颗粒的全氟溴辛烷纳米粒（Fe3O4-PFOB）增强超声显像的效果.方法 将Fe3O4-PFOB纳米粒与RAW264.7 巨噬细胞孵育30 min、1 h后,分别进行光镜及体外超声检查.12只健康SD大鼠随机分为2组,分别于注射Fe3O4-PFOB及空白PFOB纳米粒前后进行大鼠肝脏超声显像,并用DFY超声图像定量分析诊断仪评价显像效果.结果 Fe3O4-PFOB纳米粒与巨噬细胞孵育1 h后可见大量Fe3O4-PFOB纳米粒被巨噬细胞吞噬,巨噬细胞吞噬纳米粒后能够增强超声回声信号.体内显像中,与空白PFOB纳米粒相比,Fe3O4-PFOB能够更好地增强大鼠肝实质超声显像效果,而且其增强显影时间延长.结论 膜壳装载Fe3O4颗粒的Fe3O4-PFOB纳米粒能够有效增强超声显像效果,有望实现一种造影剂多种模态显像.Objective To study the feasibility of the Fe3O4-loaded lipid perfluorooctylbromide nanoparticles （Fe3O4-PFOB） for enhanced ultrasound imaging.Methods The Fe3O4-PFOB nanoparticles,incubated with RAW264.7 macrophage cells,were monitored by microscope and ultrasound.Twelve SD rats were randomized into two groups,Fe3O4-PFOB group and PFOB group.Ultrasound imaging of rats＇ liver was performed before and after intravenous injection of the contrast agents.The liver echogenic intensity was quantified by DFY ultrasound quantified system analysis.Results Incubation of the Fe3O4-PFOB nanoparticles with macrophages resulted in the uptake of Fe3O4-PFOB by macrophages.Macrophages loaded with Fe3O4-PFOB exhibited enhanced echogenicity in vitro.In in vivo imaging,Fe3O4-PFOB produced better and prolonged ultrasound enhancement of rats＇ liver compared to PFOB nanoparticles.Conclusions Fe3O4-PFOB nanoparticles could enhance ultrasound imaging and may potentially serve as a multimodal probe for ultrasound,CT and MR imaging.

关 键 词：超声检查 纳米粒子 全氟溴辛烷 超顺磁性氧化铁 

分 类 号：R445.1[医药卫生—影像医学与核医学]