针刺结合运动训练对脑梗死大鼠学习记忆功能和患侧海马CA3区微管相关蛋白-2表达的影响  

Effects of acupuncture in combination with exercise on learning, memory and the expression of microtubule- associated protein-2 in the hippocampal CA3 region after experimental focal cerebral infarction in rats

作　　者：姜伟[1] 杨敏[1] 毕研贞[1] 胡可慧[1] 余茜[2] 

机构地区：[1]泸州医学院附属医院康复医学科,泸州646000 [2]四川省人民医院康复医学科

出　　处：《中华物理医学与康复杂志》2011年第7期484-487,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的探讨针刺结合运动训练对脑梗死大鼠患侧海马CA3区微管相关蛋白-2（MAP-2）表达的影响及其促进学习记忆功能恢复的可能机制。方法将80只Wistar大鼠分为假手术组（8只）和手术组（72只），后者制成右侧大脑中动脉闭塞（MCAO）模型后再分为模型组、运动训练组、针刺运动训练组，每组24只，于术后第1，3，5周用免疫组化方法检测患侧海马CA3区MAP-2的表达，同时在术后第5周时进行学习记忆能力测评。结果假手术组MAP-2阳性纤维排列整齐，分布密集。梗死后患侧海马CA3区阳性神经元及树突纤维减少。术后1周针刺运动训练组MAP-2阳性纤维稍有增多，与模型组、运动训练组比较差异均有统计学意义（P〈0．01）；在术后第3周、5周时MAP-2阳性表达明显增多，光密度值高于运动训练组（P〈0．05）和模型组（P〈0．01）。术后第5周针刺运动训练组在Y迷宫分辨学习测试中记忆力明显优于模型组（P〈0．01）和运动训练组（P〈0．05）。结论针刺配合运动训练可明显促进脑梗死后患侧海马CA3区树突结构可塑性变化，且MAP-2表达增加与学习记忆功能恢复成正相关。Objective To study the expression of microtubule-associated protein-2 （MAP-2） in the CA3 region of the hippocampus and any effect of acupuncture combined with exercise training on the recovery of learning and memory after experimental cerebral infarction. Methods Eighty Wistar rats were randomly divided into a sham-operation group （n = 8） and an operation group （n--72） subdivided randomly and equally into a model group, an exercise training group and an acupuncture plus exercise training group, with 24 rats in each group. After right middle cerebral artery occlusion or a mock operation, the expression of MAP-2 in the CA3 region of the hippocampus was detected with immunohistochemistry 1, 3 and 5 weeks after the operation. Learning and memory function was assessed at the 5th week post operation. Results MAP-2- positive fibers were arranged in an orderly way and distributed densely in the sham- operation group. After cerebral infarction, number of MAP-2 positive neurons and dendrite fibers decreased in the CA3 region of the affected side. A week after the operation, MAP-2 positive fibers had increased significantly in the acu- puncture plus exercise group compared with the model group and the exercise group. At the 3rd and 5th week post oper- ation, expression of MAP-2 and optical density had increased significantly compared with the model group and the exer- cise training group. At the 5th week post operation, Y maze discrimination and learning tests showed that learning and memory had improved significantly more in the acupuncture plus exercise training group compared with the model group and the exercise training group. Conclusions Exercise training combined with acupuncture can promote changes in dendritic plasticity and increase MAP-2 expression in the CA3 region after cerebral infarction. This may be correlated positively with the recovery of learning and memory after cerebral infarction, at least in rats.

关 键 词：脑梗死 学习记忆 针刺 运动训练 微管相关蛋白-2 海马 

分 类 号：R285.5[医药卫生—中药学]