睡眠间歇低氧大鼠心室重塑与心肌细胞凋亡和Rho激酶表达的关系  

Involvement of sleep intermittent hypoxia in remodeling,apoptosis and expression of Rho kinase in rat myocardial cells

作　　者：佟浩[1] 张曼[2] 王实[3] 柏树令[1] 

机构地区：[1]中国医科大学基础医学院人体解剖学教研室,沈阳110001 [2]沈阳医学院奉天医院循环内科,沈阳110024 [3]沈阳医学院奉天医院呼吸内科,沈阳110024

出　　处：《解剖学报》2011年第4期470-474,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30772215);辽宁省自然科学基金资助项目(20102220)

摘　　要：目的观察睡眠中间歇低氧状态下大鼠心室重塑、血液动力学异常与心肌细胞凋亡和Rho激酶表达的关系,探讨间歇低氧与心室重塑和心力衰竭之间关系的分子机制。方法 24只雄性Wistar大鼠分3组,每组8只。给予适度限制饮食量的普通饮食和每天正常运动量游泳1h,分为常氧(A组)、间歇低氧(B组)、间歇低氧+法舒地尔(C组),60d后检测血液动力学指标、心室重量、体重、形态学检测(HE染色和凋亡细胞检测)和RT-PCR检测Rho激酶mRNA表达。结果与A组比较,B组血液动力学指标恶化,心室肥厚指数增加,心肌细胞排列紊乱,凋亡明显及Rho激酶表达增加。与B组比较,C组血液动力学指标改善,心室肥厚指数减小,心肌细胞排列紊乱减轻,凋亡减少及Rho激酶表达降低。结论间歇低氧与心室重塑及心力衰竭的发生、发展密切相关。细胞凋亡和炎症因子Rho激酶表达增加与之密切相关。Objective To explore the underlying molecular mechanisms relevant to intermittent hypnxia, remodeling and heart failure by observing the changes of remodeling, bemodynamie parameters, apoptosis and the expression of Rho kinase in the myocardial cells of rats with sleep intermittent hypoxia. Methods Male Wistar rats were given limited normal diet （20g/d per rat） and swam for 1hour at 6pro. The rats were divided into 3 groups （ n= 8 per group） ： a normal oxygen group （group A, breathing 21% O2 for 8hours per day） , an intermittent hypoxia group （ group B, breathing 10% O2 and air altered per 90 see for 8hours per day） and a fasudil group [ group C, breathing 10% O2 and air altered per 90 see for 8hours per day, 20mg/（kg · d） fasudil Qd, i. h. ]. Sixty days later, hemodynamic parameters, lefl ventricular weight and weight of rats were measured. Morphological changes （HE staining ）, apoptosis and the expression of Rho kinase mRNA in rat myocardial cells were examined. Results As enmpared to group A, group B showed abnormal hemodynamie parameters, an increase in the index of left ventrieular hypertrophy, disarrangement of myocardial cells, remarkable apoptosis, up-regulation of Rho kinase mRNA expression. These changes were improved significantly in group C in comparison with group B. Conclusion Intermittent hypoxia is associated with the pathngenesis of remodeling and heart failure. Apoptosis and Rho kinase may play a key role in remodeling and heart failure under the enndition of sleep intermittent hypoxia.

关 键 词：间歇低氧 心室重塑 RHO激酶 睡眠呼吸暂停综合征 反转录-聚合酶链式反应 大鼠 

分 类 号：R541.6[医药卫生—心血管疾病]