何首乌饮对运动性疲劳大鼠睾丸组织17β-羟基类固醇脱氢酶表达的影响  

Effect of Heshouwuyin on the expression of 17β-hydroxysteroid dehydrogenase in testis tissue of exercise-induced fatigue rats

作　　者：郭银[1] 牛嗣云[2] 高福禄[3] 曲银娥[4] 赵秀军[4] 陈雪[4] 郭凯华[1] 孙一翀[1] 

机构地区：[1]承德医学院基础医学部,河北承德067000 [2]河北大学医学部组织学胚胎学教研室,河北保定071000 [3]河北师范大学,石家庄050016 [4]河北医科大学组织学胚胎学教研室,石家庄050017

出　　处：《解剖学报》2011年第4期532-536,共5页Acta Anatomica Sinica

基　　金：河北省自然科学基金资助项目(c2009000269)

摘　　要：目的探讨何首乌饮对运动性疲劳大鼠睾丸组织17β-羟基类固醇脱氢酶(17β-HSD)的影响。方法 50只雄性SD大鼠随机分为5组,A组为安静对照组;B组为安静何首乌饮组;C组为模型组;D组为何首乌饮治疗组;E组为何首乌饮预防组,每组10只。C、D和E组大鼠复制运动性疲劳动物模型,其中E组每天训练前给予何首乌饮20g/(kg.d)(含生药9.6kg/L)灌胃作为预防。模型成功后D组大鼠给予何首乌饮20g/(kg.d)(含生药9.6kg/L)灌胃治疗60d。放射免疫法检测各组大鼠血清睾酮浓度,分别采用免疫组织化学法、Western blotting和RT-PCR检测各组大鼠睾酮合成催化酶的表达变化。结果 17β-HSD蛋白的阳性产物主要表达在睾丸间质细胞。17β-HSD mRNA及蛋白表达变化:与C组相比,D组和E组17β-HSD有显著性提高(P<0.05),而D组和E组无差异(P>0.05)。结论何首乌饮可以提高睾酮合成限速酶17β-HSD的表达。Objective To explore the effect of Heshouwuyin on the expression of 17β-hydroxysteroid dehydrogenase （17β-HSD） in testis tissue of exercise-induced fatigue rats. Methods Fifty SD rats were divided randomly into 5 groups, each group had 10 rats including normal control group（A group） , Heshouwuyin administered normal group （B group）, model control group （ C group）, Heshouwuyin treated group （ D group）, Heshouwuyin prevented group （ E group）. The rats in the C, D and E groups were made exercise-induced fatigue animal model, and animals were administrated heshouwuyin[20g/（kg.d）,contained crude drug 9.6kg/L] , with the exception of E group, animals in E group were administrated heshouwuyin before modeling. The D group was treated with Heshouwuyin by intragastrie administration for 60 days after success of modeling. The blood serum concentration of testosterone was detected by radioimmunity, and immunohistochemistry method, Western blotting and RT-PCR were used to observe the differential expression of 17β-HSD. Results 17β-HSD protein expressed in Leydig cells. Compared with C group, the expressions of 17β-HSD protein and mRNA in D, E groups increased （P 〈 0. 05） ,but there was no significant difference between D and E groups（P 〉 0.05 ）. Conclusion The expressions of 17β-HSD can be up-regulatded by Heshouwuyin.

关 键 词：何首乌饮 运动性疲劳 睾酮 17β-羟基类固醇脱氢酶 免疫组织化学 免疫印迹法 反转录-聚合酶链反应 大鼠 

分 类 号：R339.4[医药卫生—人体生理学]