小鼠肾脏发生发育中BAX的表达  

Expression of BAX during the development of mouse kidney

作　　者：宋小峰[1,2] 任昊[1] 陈雪[1] 翟效月[1] 

机构地区：[1]中国医科大学组织学与胚胎学教研室,沈阳110001 [2]辽宁医学院组织学与胚胎学教研室,辽宁锦州121001

出　　处：《解剖学报》2011年第4期548-551,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30871313);中国博士后科学基金资助项目(20070420206);中国博士后科学基金特别资助项目(200801399);高等学校博士学科点专项科研基金资助项目(200801590016);辽宁省教育厅基金资助项目(2009A454)

摘　　要：目的探讨小鼠肾脏发育过程中促凋亡蛋白BAX的表达。方法选取胚龄16、18d和出生后1、3、5、7、14、21d的小鼠共54只,应用免疫组织化学、免疫荧光技术并结合体视学分析方法观察树脂切片上小鼠不同发育时期BAX的表达。结果 BAX的表达主要出现在近端小管,随着发育的不断进行,BAX的表达由皮质的近髓质区逐渐发展到中层皮质区,最后出现在浅层皮质区。BAX的面数密度从E16d到P3d逐渐升高,随后下降,在P7d又逐渐升高。结论小鼠肾脏发育过程中BAX的表达与近端小管的发育密切相关,可能影响近端小管的存活、分化和成熟。Objective To explore the expression of BAX during mouse kidney development. Methods Fifty-four mice from embryonic day 16, 18 and postnatal day 1, 3, 5, 7, 14, 21 were chosen to investigate the expression of BAX on epoxy sections of different development phases of mouse kidney with immunohistochemistry technique and stereologieal methods. Results The immunohistochemical positive staining of BAX was mainly observed in proximal tubules. During development, the expressive position of BAX gradually moved from deep to middle cortex, and finally was located on the superficial cortex. The numerical area density of BAX gradually increased from E16d to P3d, then descend, and turned to increase again at postnatal day 7. Conclusion During the development of kidney, the expression of BAX closely relates to the growth of proximal tubules, and may impact the proximal tubules in surviving, differentiating and maturing.

关 键 词：BAX 肾脏 发生发育 树脂切片 体视学 免疫组织化学 免疫荧光 小鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]